Cresol Red: Difference between revisions
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Revision as of 03:45, 14 January 2009
Cresol red is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.
Agarose molecular weight marker
advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue
- molecular weight 404 g/mol
- in 1% agarose at ~125 bp
- in 2% agarose at ~300 bp (between xylene cyanol and bromophenol blue)
pH indicator
- amber - acidic pH
- yellow - neutral
- red - alkaline