Cresol Red

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(molecular weight, pH indicator)
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[[Image:Cresol red.png|right|150px]]
[[Image:Cresol red.png|right|150px]]
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'''Cresol red''' is a pH indicator and molecular weight marker for agarose gels.
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'''Cresol red''' is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.
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== Agarose molecular weight marker ==
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advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue
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* molecular weight 404 g/mol
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* in 1% agarose at ~125 bp
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* in 2% agarose at ~300 bp (between xylene cyanol and bromophenol blue)
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== pH indicator ==
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* amber - acidic pH
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* yellow - neutral
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* red - alkaline
== External links ==
== External links ==

Revision as of 05:44, 14 January 2009

Cresol red is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.

Agarose molecular weight marker

advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue

  • molecular weight 404 g/mol
  • in 1% agarose at ~125 bp
  • in 2% agarose at ~300 bp (between xylene cyanol and bromophenol blue)

pH indicator

  • amber - acidic pH
  • yellow - neutral
  • red - alkaline

External links

Personal tools