Create running gel: Difference between revisions
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To create 10ml of Running gel | |||
{| border="1" | |||
|+ To create 10ml of Running gel | |||
! Material !! 7.5% !! 9% !! 10% !! 11% !! 12.5% | |||
|- | |||
! 30% Acrylamide | |||
| 2.5 || 3 || 3.3 || 3.6 || 4.2 | |||
|- | |||
! 1.5M Tris pH8.8 | |||
| 2.5 || 2.5 || 2.5 || 2.5 || 2.5 | |||
|- | |||
! 10%SDS | |||
| 100ul || 100ul || 100ul || 100ul || 100ul | |||
|- | |||
! 10%APS | |||
| 100ul || 100ul || 100ul || 100ul || 100ul | |||
|- | |||
! TEMED | |||
| 10ul || 10ul || 10ul || 10ul || 10ul | |||
|- | |||
! Water | |||
| 4.8 || 4.3 || 4 || 3.7 || 3.1 | |||
|- | |||
|} | |||
Latest revision as of 10:40, 6 September 2006
| Material | 7.5% | 9% | 10% | 11% | 12.5% |
|---|---|---|---|---|---|
| 30% Acrylamide | 2.5 | 3 | 3.3 | 3.6 | 4.2 |
| 1.5M Tris pH8.8 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 |
| 10%SDS | 100ul | 100ul | 100ul | 100ul | 100ul |
| 10%APS | 100ul | 100ul | 100ul | 100ul | 100ul |
| TEMED | 10ul | 10ul | 10ul | 10ul | 10ul |
| Water | 4.8 | 4.3 | 4 | 3.7 | 3.1 |
