Corum:Protocols: Difference between revisions
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# [http://openwetware.org/wiki/Corum:DNA_Quantification DNA Quantification by Quantifluore] | # [http://openwetware.org/wiki/Corum:DNA_Quantification DNA Quantification by Quantifluore] | ||
# DNA Linker Design | # DNA Linker Design | ||
# DNA Digestion by Restriction Enzymes | # [http://openwetware.org/wiki/Corum:Digestion DNA Digestion by Restriction Enzymes] | ||
# [http://openwetware.org/wiki/Corum:DNA_Hybridization Linker Construction by ssDNA Hybridization] | # [http://openwetware.org/wiki/Corum:DNA_Hybridization Linker Construction by ssDNA Hybridization] | ||
# [http://openwetware.org/wiki/Corum:T4_Ligation DNA Ligation by T4 Ligase] | # [http://openwetware.org/wiki/Corum:T4_Ligation DNA Ligation by T4 Ligase] | ||
Revision as of 11:17, 29 June 2012
Overview
Protocols for the Noireaux Lab at the University of Minnesota [Physics Department http://www.physics.umn.edu].
Protocols
- Cell Plating
- Miniculture
- Competent Cells
- Transformation
- Gel electrophoresis
- Miniprep
- DNA Purification by PCR Purification Kit
- Gel Extraction of DNA
- DNA Quantification by Quantifluore
- DNA Linker Design
- DNA Digestion by Restriction Enzymes
- Linker Construction by ssDNA Hybridization
- DNA Ligation by T4 Ligase
- Polymerase Chain Reaction (PCR)
- Whole Plasmid PCR
- DNA Sequencing
- Cell Free Expression in Batch Mode
- Cell Free Expression in Continuous Mode
- Cell Free Expression in Phospholipid Vesicles
- Plaque Counting
Contact
Digital Signature
SC 16:08, 25 June 2012 (EDT)
