Corum:Protocols: Difference between revisions
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==Contact== | ==Contact== | ||
* [http://openwetware.org/wiki/User:Sean_P_Corum Sean P Corum] | * [http://openwetware.org/wiki/User:Sean_P_Corum Sean P Corum] | ||
==Digital Signature== | ==Digital Signature== |
Revision as of 14:45, 25 June 2012
Overview
Protocols for the Noireaux Lab at the University of Minnesota [Physics Department http://www.physics.umn.edu].
Protocols
- Cell Plating
- Miniculture
- Competent Cells
- Transformation
- Gel electrophoresis
- Miniprep
- DNA Purification by PCR Purification Kit
- Gel Extraction of DNA
- DNA Quantification by Quantifluore
- DNA Linker Design
- DNA Digestion by Restriction Enzymes
- Linker Construction by ssDNA Hybridization
- DNA Ligation by T4 Ligase
- Polymerase Chain Reaction (PCR)
- Whole Plasmid PCR
- DNA Sequencing
- Cell Free Expression in Batch Mode
- Cell Free Expression in Continuous Mode
- Cell Free Expression in Phospholipid Vesicles
Contact
Digital Signature
SC 16:08, 25 June 2012 (EDT)