Corum:DNA Sequencing

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Revision as of 13:33, 25 June 2012 by Sean P Corum (talk | contribs) (New page: ==Overview== DNA Sequencing of miniprep DNA plasmid product at the [http://www.umn.edu University of Minnesota] [http://www.bmgc.umn.edu BioMedical Genomics Center]. ==Materials== * 5 μ...)
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Overview

DNA Sequencing of miniprep DNA plasmid product at the University of Minnesota BioMedical Genomics Center.

Materials

  • 5 μL miniprep plasmid DNA product
  • 4 μL H2O
  • 3 μL 2μM sequencing primer

Procedure

  1. Combine components into a 1.5 mL tube. Label tubes XYZ1, ZYZ2, ..., where XYZ are your initials.
  2. Fill out and print the DNA sequencing form for individual tubes. Order details - Template: plasmid, Template mass: 500 ng, Template length: <length of plasmid>, Sequencing option: Sanger classic, Hardcopies: no hardcopies.
  3. Place the sample tubes and form into a plastic bag.
  4. Deposit at a BMGC sequencing drop off site.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

Contact

or instead, discuss this protocol.


Digital Signature

  • SC 16:33, 25 June 2012 (EDT):
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