Corum:DNA Hybridization

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SC 20:55, 19 June 2012 (EDT):

Contents

Overview

Hybridization of ssDNA oligonucleotides.

Materials

  • 5 μL 2mM sense oligonucleotide
  • 5 μL 2mM antisense oligonucleotide

Procedure

  1. Combine sense and antisense oligonucleotides. Vortex and spin.
  2. Seal tube airtight with paraffin and place in weighted holder.
  3. Boil 400 mL water in a 500 mL beaker.
  4. Cool on bench 5 min.
  5. Place weighted holder with sealed oligonucleotides into the beaker.
  6. Incubate on bench overnight or until water is about room temperature.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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References

Relevant papers, books, and websites

  1. New England Biolabs

Contact

or instead, discuss this protocol.

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