Cong T. Trinh:electrophoresis: Difference between revisions
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(New page: =<div class="center" style="width:auto; margin-left:auto; margin-right:auto;"> DNA Gel Electrophoresis </div>= ==Gel Preparation== *60mL of 1x TAE Buffer '''The amount of agarose to use i...) |
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*.54g is typically used for the small gels, 1.08g for the large gels | |||
-Microwave the Agarose and TAE buffer mixture for ~1:15 min. | -Microwave the Agarose and TAE buffer mixture for ~1:15 min. | ||
*3.5-6 μL of Ethidium Bromide | *3.5-6 μL of Ethidium Bromide | ||
Revision as of 15:08, 30 November 2011
DNA Gel Electrophoresis
Gel Preparation
- 60mL of 1x TAE Buffer
The amount of agarose to use in your gel depends on the DNA in question. Use the following table as a rough guide:
| Agarose Concentration (g/100mL) | Optimal DNA Resolution (kb) |
|---|---|
| 0.5 | 1 - 30 |
| 0.7 | 0.8 - 12 |
| 1.0 | 0.5 - 10 |
| 1.2 | 0.4 - 7 |
| 1.5 | 0.2 - 3 |
- .54g is typically used for the small gels, 1.08g for the large gels
-Microwave the Agarose and TAE buffer mixture for ~1:15 min.
- 3.5-6 μL of Ethidium Bromide
