Colony PCR - Revision history

Christopher C Vanlang at 10:39, 1 July 2012

Christopher C Vanlang — Sun, 01 Jul 2012 10:39:55 GMT

Torsten Waldminghaus — Mon, 23 Feb 2009 17:03:25 GMT

Reshma P. Shetty — Tue, 08 May 2007 21:18:23 GMT

Reshma P. Shetty — Tue, 08 May 2007 21:16:54 GMT

←Older revision		Revision as of 21:16, 8 May 2007
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	#I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.		#I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.

-	[[Category:~~Escherichia coli~~]] [[Category:~~Protocol~~]] [[Category:DNA]]	+	[[Category:Protocol]]
		+	[[Category:In vivo]]
		+	[[Category:Escherichia coli]]
		+	[[Category:DNA]]

Reshma P. Shetty — Fri, 27 Apr 2007 19:30:00 GMT

←Older revision		Revision as of 19:30, 27 April 2007
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	#I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.		#I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.

-	[[Category:Escherichia coli]] [[Category:Protocol]]	+	[[Category:Escherichia coli]] [[Category:Protocol]] [[Category:DNA]]

Reshma P. Shetty — Fri, 20 Apr 2007 17:41:42 GMT

←Older revision		Revision as of 17:41, 20 April 2007
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	==Notes==		==Notes==
	#I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.		#I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.
		+
		+	[[Category:Escherichia coli]] [[Category:Protocol]]

JulianEskin — Wed, 21 Sep 2005 21:09:21 GMT

←Older revision		Revision as of 21:09, 21 September 2005
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	*[[Endy:Colony PCR]]		*[[Endy:Colony PCR]]
	*[[Knight:Colony PCR]]		*[[Knight:Colony PCR]]
-	*[[Silver: Colony PCR]]

	==Notes==		==Notes==
	#I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.		#I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.

JulianEskin — Wed, 21 Sep 2005 21:08:07 GMT

←Older revision		Revision as of 21:08, 21 September 2005
Line 6:		Line 6:
	*[[Endy:Colony PCR]]		*[[Endy:Colony PCR]]
	*[[Knight:Colony PCR]]		*[[Knight:Colony PCR]]
		+	*[[Silver: Colony PCR]]

	==Notes==		==Notes==
	#I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.		#I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.

Barry Canton — Thu, 14 Jul 2005 16:12:49 GMT

Specific Protocols

←Older revision		Revision as of 16:12, 14 July 2005
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	==Specific Protocols==		==Specific Protocols==
	*[[Endy:Colony PCR]]		*[[Endy:Colony PCR]]
-	*[[Knight:Colony PCR]].	+	*[[Knight:Colony PCR]]

	==Notes==		==Notes==
	#I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.		#I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.

Barry Canton — Thu, 14 Jul 2005 16:12:19 GMT

Description

←Older revision		Revision as of 16:12, 14 July 2005
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-	==~~Description~~==	+	==General Information==

	Colony PCR is can be used after a transformation to screen colonies for the desired plasmid. Primers are used which generate a PCR product of known size. Thus, any colonies which give rise to an amplification product of the expected size are likely to contain the correct DNA sequence.		Colony PCR is can be used after a transformation to screen colonies for the desired plasmid. Primers are used which generate a PCR product of known size. Thus, any colonies which give rise to an amplification product of the expected size are likely to contain the correct DNA sequence.