Colony PCR

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(Description)
(Specific Protocols)
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==Specific Protocols==
==Specific Protocols==
*[[Endy:Colony PCR]]
*[[Endy:Colony PCR]]
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*[[Knight:Colony PCR]].
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*[[Knight:Colony PCR]]
==Notes==
==Notes==
#I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.
#I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.

Revision as of 11:12, 14 July 2005

General Information

Colony PCR is can be used after a transformation to screen colonies for the desired plasmid. Primers are used which generate a PCR product of known size. Thus, any colonies which give rise to an amplification product of the expected size are likely to contain the correct DNA sequence.

Specific Protocols

Notes

  1. I have compared Taq and Vent using this reaction mix and found that Taq worked better. For very long amplicons, I would recommend using Phusion, which has its own reaction mix. I have not found a difference between Phusion and Taq on amplicons shorter than ~3kb.
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