Citizen Science/Open Fluorometer Project/Resources: Difference between revisions
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* "An Inexpensive LED-Based Fluorometer Used to Study a Hairpin-Based DNA Nanomachine", Hanwen Yan, 2005. | * "An Inexpensive LED-Based Fluorometer Used to Study a Hairpin-Based DNA Nanomachine", Hanwen Yan, 2005. | ||
==Selected designs (in details)== | |||
* [[Citizen_Science/Open_Fluorometer_Project/Resources/Novak_2007_Fluorometer | Novak's design (2007)]] | |||
* [[Citizen_Science/Open_Fluorometer_Project/Resources/Shrinivasan_2006_Fluorometer | Shrinivasan's design (2006)]] | |||
* [[Citizen_Science/Open_Fluorometer_Project/Resources/Yang_2009_Fluorometer | Yang's design (2009)]] | |||
==Optics== | ==Optics== | ||
Revision as of 06:34, 28 June 2009
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Resources
Vincent 10:05, 6 June 2009 (EDT): to be updated over the summer 2009 Feel free to add more relevant resources.
Fluorescence measurements and standards
- Great tutorial for beginners
- Tutorial on Fluorescence and Fluorescent Instrumentation (PDF from Univ Washington)
- Standardization of Fluorescence Measurements: Criteria for the Choice of Suitable Standards and Approaches to Fit-for-Purpose Calibration Tools
- Standardization and Quality Assurance in Fluorescence Measurements: Techniques (Book)
Reviews
- Xiao D, Zhao S, Yuan H, and Yang X. CE detector based on light-emitting diodes. Electrophoresis. 2007 Jan;28(1-2):233-42. DOI:10.1002/elps.200600473 |
- Xiao D, Yan L, Yuan H, Zhao S, Yang X, and Choi MM. CE with LED-based detection: an update. Electrophoresis. 2009 Jan;30(1):189-202. DOI:10.1002/elps.200800415 |
Interesting published designs
- Yang FB, Pan JZ, Zhang T, and Fang Q. A low-cost light-emitting diode induced fluorescence detector for capillary electrophoresis based on an orthogonal optical arrangement. Talanta. 2009 May 15;78(3):1155-8. DOI:10.1016/j.talanta.2009.01.033 |
- Novak L, Neuzil P, Pipper J, Zhang Y, and Lee S. An integrated fluorescence detection system for lab-on-a-chip applications. Lab Chip. 2007 Jan;7(1):27-9. DOI:10.1039/b611745g |
- Shrinivasan S, Norris PM, Landers JP, and Ferrance JP. A low-cost, low-power consumption, miniature laser-induced fluorescence system for DNA detection on a microfluidic device. Clin Lab Med. 2007 Mar;27(1):173-81. DOI:10.1016/j.cll.2006.12.010 |
- Segura-Carretero A, Fernández-Sánchez JF, and Fernández-Gutiérrez A. Simple luminescence detector for capillary electrophoresis. Methods Mol Biol. 2009;503:221-37. DOI:10.1007/978-1-60327-567-5_12 |
- "An Inexpensive LED-Based Fluorometer Used to Study a Hairpin-Based DNA Nanomachine", Hanwen Yan, 2005.
Selected designs (in details)
Optics
- Equipment: lenses, dichroic mirrors, optical filters
- Thorlabs (great quality, but expensive)
- check on ebay
Electronics
- Equipment: LEDs, microcontroller
Commercial products
- Qubit from Invitrogen
- TBS-380 Mini-Fluorometer from Turner BioSystems
- SpectroFluorometer from Stellar Net Inc.
- Nanodrop Fluorometer
