Citizen Science/Open Colony Counter Project
From OpenWetWare
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Driving forces:
Team:
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Specifications
Features
- General:
- can detect and count microbial colonies
- accepted plate format: 60-90mm plates or multi-well plate format
- desktop device for the wetlab bench
- requires a PC (windows, osx, or linux)
- lab automation friendly design
- Open Source design
- Available Measurements:
- colony count
- individual colony properties (size, roundness, position)
- export in CSV format or local database
- PDF/HTML report with overlaid image with detection results + colony statistics
- Image processing:
- one-step processing for the user
- colony count in less than 3 seconds.
- automatic background detection
- utilizes LED illumination to optimize contrast between colonies and substrate.
- possible manual colony count editing for improved count.
- USB Webcam as an image acquisition device
Technological stack under investigation:
- Image acquisition device: USB Webcam-based
- Light source: white LEDs, 470nm LEDs for GFP-like detection
- Image processing engine: OpenCV library