Citizen Science/Open Colony Counter Project

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===Features===
===Features===
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* General
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* '''General:'''
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** bacterial colonies or DAPI stained mammalian cells
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** can detect and count microbial colonies
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**  
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** accepted plate format: 60-90mm plates or multi-well plate format
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* Image processing-related
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** desktop device for the wetlab bench
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** Colony count in less than 3 seconds.
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** requires a PC (windows, osx, or linux)
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*  
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** lab automation friendly design
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* Open source design
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** Open Source design
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* '''Available Measurements:'''
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** colony count
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** individual colony properties (size, roundness, position)
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** export in CSV format or local database
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** PDF/HTML report with overlaid image with detection results + colony statistics
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* '''Image processing:'''
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** one-step processing for the user
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** colony count in less than 3 seconds.
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** automatic background detection
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** utilizes LED illumination to optimize contrast between colonies and substrate.
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** possible manual colony count editing for improved count.
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** USB Webcam as an image acquisition device
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==Technological stack under investigation:==
* Image acquisition device: USB Webcam-based
* Image acquisition device: USB Webcam-based
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* Light sources: white LEDs
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* Light source: white LEDs, 470nm LEDs for GFP-like detection
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* Image processing engine: OpenCV library-based
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* Image processing engine: OpenCV library
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* Calibration Mode / Automatic Mode
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* Colony Counting mode / Colony Picking mode (if interfaced with a robotic platform)
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* Lab automation friendly design
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===Optional Features===
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===Requirements===
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Revision as of 12:58, 1 August 2010

Open Colony Counter Project

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visit counter for blogspot

Driving forces:

  • to provide an open source colony counter system
  • to ease access to quantitative equipment in Life Sciences
  • to deliver a low-cost / easy to reproduce design

Team:

Synthetic Biology Foundations Prism

Specifications

Features

  • General:
    • can detect and count microbial colonies
    • accepted plate format: 60-90mm plates or multi-well plate format
    • desktop device for the wetlab bench
    • requires a PC (windows, osx, or linux)
    • lab automation friendly design
    • Open Source design
  • Available Measurements:
    • colony count
    • individual colony properties (size, roundness, position)
    • export in CSV format or local database
    • PDF/HTML report with overlaid image with detection results + colony statistics
  • Image processing:
    • one-step processing for the user
    • colony count in less than 3 seconds.
    • automatic background detection
    • utilizes LED illumination to optimize contrast between colonies and substrate.
    • possible manual colony count editing for improved count.
    • USB Webcam as an image acquisition device

Technological stack under investigation:

  • Image acquisition device: USB Webcam-based
  • Light source: white LEDs, 470nm LEDs for GFP-like detection
  • Image processing engine: OpenCV library
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