Citizen Science/Open Colony Counter Project
From OpenWetWare
(Difference between revisions)
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==Specifications== | ==Specifications== | ||
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| + | [[User:Vincent Rouilly|Vincent]] 13:06, 1 August 2010 (EDT): feel free to comment. | ||
===Features=== | ===Features=== | ||
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** desktop device for the wetlab bench | ** desktop device for the wetlab bench | ||
** requires a PC (windows, osx, or linux) | ** requires a PC (windows, osx, or linux) | ||
| + | ** Laser-cut design for the casing | ||
** lab automation friendly design | ** lab automation friendly design | ||
** Open Source design | ** Open Source design | ||
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** possible manual colony count editing for improved count. | ** possible manual colony count editing for improved count. | ||
** USB Webcam as an image acquisition device | ** USB Webcam as an image acquisition device | ||
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| + | ==User case: typical use== | ||
| + | # the user places his/her plate inside the colony counter | ||
| + | # the user opens the colony counting application on the attached PC | ||
| + | # the user triggers the image acquistion and counting by pressing a single button | ||
| + | # results are displayed as an overlay on the acquired image | ||
| + | # if required, the user can adapt image processing parameters to improve detection | ||
| + | # the user can then export results in various format (CSV, HTML, PDF) | ||
==Technological stack under investigation:== | ==Technological stack under investigation:== | ||
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* Light source: white LEDs, 470nm LEDs for GFP-like detection | * Light source: white LEDs, 470nm LEDs for GFP-like detection | ||
* Image processing engine: OpenCV library | * Image processing engine: OpenCV library | ||
| + | * Laser cut design for the casing | ||
| + | [[User:Vincent Rouilly|Vincent]] 13:06, 1 August 2010 (EDT): feel free to comment. | ||
Revision as of 13:06, 1 August 2010
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Driving forces:
Team:
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Specifications
Vincent 13:06, 1 August 2010 (EDT): feel free to comment.
Features
- General:
- can detect and count microbial colonies
- accepted plate format: 60-90mm plates or multi-well plate format
- desktop device for the wetlab bench
- requires a PC (windows, osx, or linux)
- Laser-cut design for the casing
- lab automation friendly design
- Open Source design
- Available Measurements:
- colony count
- individual colony properties (size, roundness, position)
- export in CSV format or local database
- PDF/HTML report with overlaid image with detection results + colony statistics
- Image processing:
- one-step processing for the user
- colony count in less than 3 seconds.
- automatic background detection
- utilizes LED illumination to optimize contrast between colonies and substrate.
- possible manual colony count editing for improved count.
- USB Webcam as an image acquisition device
User case: typical use
- the user places his/her plate inside the colony counter
- the user opens the colony counting application on the attached PC
- the user triggers the image acquistion and counting by pressing a single button
- results are displayed as an overlay on the acquired image
- if required, the user can adapt image processing parameters to improve detection
- the user can then export results in various format (CSV, HTML, PDF)
Technological stack under investigation:
- Image acquisition device: USB Webcam-based
- Light source: white LEDs, 470nm LEDs for GFP-like detection
- Image processing engine: OpenCV library
- Laser cut design for the casing
