Christian Niederauer/Notebook/RacingBacteria/Protocols/Microfluidics/PDMS: Difference between revisions
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(New page: =How to Create a PDMS-Stamp from Silicone/Araldite Mold= ==Preparation of PDMS mixture== * with pipette boy and 5ml stripette pour ~4g of elastomer into a cup * with micropipette (1000µl...) |
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==Final Processing== | ==Final Processing== | ||
* punch holes with 2mm coring tool at the desired connection points under dissection microscope (tip: target the hole with coring tool under microscope, then put the stamp onto one finger with alufoil between stamp and finger and push/turn until you feel the coring tool on your skin) | * punch holes with 2mm coring tool at the desired connection points under dissection microscope (tip: target the hole with coring tool under microscope, then put the stamp onto one finger with alufoil between stamp and finger and push/turn until you feel the coring tool on your skin) | ||
* [[Christian Niederauer/Notebook/RacingBacteria/Protocols/Plasma_Cleaning | plasma | * [[Christian Niederauer/Notebook/RacingBacteria/Protocols/Plasma_Cleaning | plasma clean]] the treated face of the stamp and a coverslip and press them together with alufoil in order to prevent contamination | ||
| Line 24: | Line 24: | ||
'''BEFORE PUNCHING HOLES''' | '''BEFORE PUNCHING HOLES''' | ||
* take a slice of PDMS with bigger or same area than the stamp | * take a slice of PDMS with bigger or same area than the stamp | ||
* [[Christian Niederauer/Notebook/RacingBacteria/Protocols/Plasma_Cleaning | plasma | * [[Christian Niederauer/Notebook/RacingBacteria/Protocols/Plasma_Cleaning | plasma clean]] unshaped face of stamp and extra slice and stick them together | ||
* now punch desired holes with 2mm coring tool | * now punch desired holes with 2mm coring tool | ||
Revision as of 07:42, 23 August 2014
How to Create a PDMS-Stamp from Silicone/Araldite Mold
Preparation of PDMS mixture
- with pipette boy and 5ml stripette pour ~4g of elastomer into a cup
- with micropipette (1000µl) add one tenth of elastomer mass (~0.4g) of linker fluid to the cup
- mix well with stripette
- degass for 30min-60min
Preparation of PDMS membrane
- pour degassed mixture onto wafer placed in petri dish with aluminium foil
- pour just enough to cover the waver
- use Leveling Petri Dish to ensure constant thickness
- araldite wafer: cure it for 3-4 hours in oven at 60°C
- silicon wafer: cure it for 2 hours in oven at 80°C for
- peel off membrane from wafer surface with forceps
- cut out the pattern but leave enough space around it
Final Processing
- punch holes with 2mm coring tool at the desired connection points under dissection microscope (tip: target the hole with coring tool under microscope, then put the stamp onto one finger with alufoil between stamp and finger and push/turn until you feel the coring tool on your skin)
- plasma clean the treated face of the stamp and a coverslip and press them together with alufoil in order to prevent contamination
If the stamp is too thin
BEFORE PUNCHING HOLES
- take a slice of PDMS with bigger or same area than the stamp
- plasma clean unshaped face of stamp and extra slice and stick them together
- now punch desired holes with 2mm coring tool
