Christian Niederauer/Notebook/RacingBacteria/Protocols/Bacteria/Streaking Bacteria: Difference between revisions
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(New page: ==Streaking Bacteria onto Agar Plate== * Petri dishes filled with 20ml of molten (80°C) 1.5% Agar, cool for 15 minutes * with inoculator loop: dip into liquid culture of [[ | revived bact...) |
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==Streaking Bacteria onto Agar Plate== | ==Streaking Bacteria onto Agar Plate== | ||
* Petri dishes filled with 20ml of molten (80°C) 1.5% Agar, cool for 15 minutes | * Petri dishes filled with 20ml of molten (80°C) 1.5% Agar, cool for 15 minutes | ||
* with inoculator loop: dip into liquid culture of [[ | revived bacteria]] and spread one line | * with inoculator loop: dip into liquid culture of [[Christian Niederauer/Notebook/RacingBacteria/Protocols/Bacteria/Glycerol_Protocol | revived bacteria]] and spread one line | ||
* after initial line, burn inoculator ring | * after initial line, burn inoculator ring & let it cool | ||
* dip ring into initial line and spread bacteria diagonally | * dip ring into initial line and spread bacteria diagonally | ||
* burn ring again | * burn ring again | ||
* repeat ... | * repeat ... | ||
<gallery>Image:Streak.gif | <gallery>Image:Streak.gif | [http://www.personal.psu.edu/faculty/k/h/khb4/enve301/301labs/301labgraphics/streak.gif source]</gallery> | ||
[http://www.personal.psu.edu/faculty/k/h/khb4/enve301/301labs/301labgraphics/streak.gif source] | |||
Latest revision as of 11:11, 23 August 2014
Streaking Bacteria onto Agar Plate
- Petri dishes filled with 20ml of molten (80°C) 1.5% Agar, cool for 15 minutes
- with inoculator loop: dip into liquid culture of revived bacteria and spread one line
- after initial line, burn inoculator ring & let it cool
- dip ring into initial line and spread bacteria diagonally
- burn ring again
- repeat ...
