Christian Niederauer/Notebook/RacingBacteria/Protocols/Bacteria/Glycerol Protocol
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Reviving Bacteria from Glycerol Stock
- Scrape the frozen surface of the culture with a sterile inoculating needle, and then immediately [Christian Niederauer/Notebook/RacingBacteria/Protocols/Bacteria/Spreading_Bacteria streak the bacteria] that adhere to the needle onto the surface of an LB agar plate containing the appropriate antibiotics. Incubate the plate overnight at 37°C
- Scrape the frozen surface of the culture with a sterile inoculating needle or tip, and immediately immerse it in 2ml growth media within a 2059 snap-cap tube. Grow the bacteria overnight in a 37°C shaker.
- Return the frozen culture to storage at -70°C
Source: doi:10.1101/pdb.prot4452
