Mode of Action
Chloramphenicol is a bacteriostatic agent that binds to the 50S ribosome and inhibits ribosomal peptide bond formation. It is sometimes uses as a way of "amplifying" plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue.
Working Concentrations and Stock Solutions
High copy plasmids allow 35 μg/ml concentration. Stock solutions can be made at 35 mg/ml in ethanol, kept at -20C.
The cat gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it. The gene is sometimes specifed as CmR or CamR.