Chloramphenicol - Revision history

Roberto Rosati at 01:52, 13 April 2010

Roberto Rosati — Tue, 13 Apr 2010 01:52:31 GMT

←Older revision		Revision as of 01:52, 13 April 2010
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	==Working Concentrations and Stock Solutions==		==Working Concentrations and Stock Solutions==
-	High copy plasmids allow 35 μg/ml concentration. Stock solutions can be made at 35 mg/ml in ethanol, kept at -20C.	+	High copy plasmids allow 35 μg/ml concentration. Low-copy plasmids like [[bacterial artificial chromosomes]] allow 12.5 μg/ml. Stock solutions can be made at 35 mg/ml in ethanol, kept at -20C.

	==Resistance Gene==		==Resistance Gene==
	The ''cat'' gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it.		The ''cat'' gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it.
	The gene is sometimes specifed as ''Cm<sup>R</sup>'' or ''Cam<sup>R</sup>''.		The gene is sometimes specifed as ''Cm<sup>R</sup>'' or ''Cam<sup>R</sup>''.
		+
		+	==Links==
		+	[http://bacpac.chori.org/clone-handling.htm Children's Hospital Oakland Research Institute (CHORI) - BAC/PAC resources]

	[[Category:Material]] [[Category:Antibiotic]]		[[Category:Material]] [[Category:Antibiotic]]

ClarkeS: typo

ClarkeS — Thu, 13 Sep 2007 16:16:22 GMT

typo

←Older revision		Revision as of 16:16, 13 September 2007
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	==Mode of Action==		==Mode of Action==
-	Chloramphenicol is a bacteriostatic agent that binds to the 50S ~~ribosome~~ and inhibits ribosomal peptide bond formation. It is sometimes ~~uses~~ as a way of "amplifying" plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue.	+	Chloramphenicol is a bacteriostatic agent that binds to the 50S ribosomal subunit and inhibits ribosomal peptide bond formation. It is sometimes used as a way of "amplifying" plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue.

	==Working Concentrations and Stock Solutions==		==Working Concentrations and Stock Solutions==

Reshma P. Shetty at 17:45, 26 July 2007

Reshma P. Shetty — Thu, 26 Jul 2007 17:45:16 GMT

←Older revision		Revision as of 17:45, 26 July 2007
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	The ''cat'' gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it.		The ''cat'' gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it.
	The gene is sometimes specifed as ''Cm<sup>R</sup>'' or ''Cam<sup>R</sup>''.		The gene is sometimes specifed as ''Cm<sup>R</sup>'' or ''Cam<sup>R</sup>''.
		+
		+	[[Category:Material]] [[Category:Antibiotic]]

Austin J. Che at 19:24, 27 April 2007

Austin J. Che — Fri, 27 Apr 2007 19:24:56 GMT

←Older revision		Revision as of 19:24, 27 April 2007
Line 3:		Line 3:

	==Working Concentrations and Stock Solutions==		==Working Concentrations and Stock Solutions==
-	High copy plasmids allow 35 μg/ml concentration. ~~The stock solution is~~ 35 mg/ml in ethanol, kept at -20C.	+	High copy plasmids allow 35 μg/ml concentration. Stock solutions can be made at 35 mg/ml in ethanol, kept at -20C.
-		+
-	~~==Plate Color Code==~~	+
-	~~Green Stripe~~	+

	==Resistance Gene==		==Resistance Gene==
	The ''cat'' gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it.		The ''cat'' gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it.
	The gene is sometimes specifed as ''Cm<sup>R</sup>'' or ''Cam<sup>R</sup>''.		The gene is sometimes specifed as ''Cm<sup>R</sup>'' or ''Cam<sup>R</sup>''.

Tk at 01:47, 18 December 2005

Tk — Sun, 18 Dec 2005 01:47:05 GMT

New page

==Mode of Action==
Chloramphenicol is a bacteriostatic agent that binds to the 50S ribosome and inhibits ribosomal peptide bond formation. It is sometimes uses as a way of "amplifying" plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue.

==Working Concentrations and Stock Solutions==
High copy plasmids allow 35 μg/ml concentration. The stock solution is 35 mg/ml in ethanol, kept at -20C.

==Plate Color Code==
Green Stripe

==Resistance Gene==
The ''cat'' gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it.
The gene is sometimes specifed as ''Cm<sup>R</sup>'' or ''Cam<sup>R</sup>''.