Chloramphenicol: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
m (typo) |
||
| Line 1: | Line 1: | ||
==Mode of Action== | ==Mode of Action== | ||
Chloramphenicol is a bacteriostatic agent that binds to the 50S | Chloramphenicol is a bacteriostatic agent that binds to the 50S ribosomal subunit and inhibits ribosomal peptide bond formation. It is sometimes used as a way of "amplifying" plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue. | ||
==Working Concentrations and Stock Solutions== | ==Working Concentrations and Stock Solutions== | ||
Revision as of 09:16, 13 September 2007
Mode of Action
Chloramphenicol is a bacteriostatic agent that binds to the 50S ribosomal subunit and inhibits ribosomal peptide bond formation. It is sometimes used as a way of "amplifying" plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue.
Working Concentrations and Stock Solutions
High copy plasmids allow 35 μg/ml concentration. Stock solutions can be made at 35 mg/ml in ethanol, kept at -20C.
Resistance Gene
The cat gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it. The gene is sometimes specifed as CmR or CamR.
