Chloramphenicol

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==Mode of Action==
==Mode of Action==
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Chloramphenicol is a bacteriostatic agent that binds to the 50S ribosome and inhibits ribosomal peptide bond formation.  It is sometimes uses as a way of "amplifying" plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue.
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Chloramphenicol is a bacteriostatic agent that binds to the 50S ribosomal subunit and inhibits ribosomal peptide bond formation.  It is sometimes used as a way of "amplifying" plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue.
==Working Concentrations and Stock Solutions==
==Working Concentrations and Stock Solutions==
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High copy plasmids allow 35 μg/ml concentration. Stock solutions can be made at 35 mg/ml in ethanol, kept at -20C.
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High copy plasmids allow 35 μg/ml concentration. Low-copy plasmids like [[bacterial artificial chromosomes]] allow 12.5 μg/ml. Stock solutions can be made at 35 mg/ml in ethanol, kept at -20C.
==Resistance Gene==
==Resistance Gene==
The ''cat'' gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it.
The ''cat'' gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it.
The gene is sometimes specifed as ''Cm<sup>R</sup>'' or ''Cam<sup>R</sup>''.
The gene is sometimes specifed as ''Cm<sup>R</sup>'' or ''Cam<sup>R</sup>''.
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==Links==
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[http://bacpac.chori.org/clone-handling.htm Children's Hospital Oakland Research Institute (CHORI) - BAC/PAC resources]
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[[Category:Material]] [[Category:Antibiotic]]

Current revision

Contents

Mode of Action

Chloramphenicol is a bacteriostatic agent that binds to the 50S ribosomal subunit and inhibits ribosomal peptide bond formation. It is sometimes used as a way of "amplifying" plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue.

Working Concentrations and Stock Solutions

High copy plasmids allow 35 μg/ml concentration. Low-copy plasmids like bacterial artificial chromosomes allow 12.5 μg/ml. Stock solutions can be made at 35 mg/ml in ethanol, kept at -20C.

Resistance Gene

The cat gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it. The gene is sometimes specifed as CmR or CamR.

Links

Children's Hospital Oakland Research Institute (CHORI) - BAC/PAC resources

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