Chloramphenicol: Difference between revisions
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==Mode of Action== | ==Mode of Action== | ||
Chloramphenicol is a bacteriostatic agent that binds to the 50S | Chloramphenicol is a bacteriostatic agent that binds to the 50S ribosomal subunit and inhibits ribosomal peptide bond formation. It is sometimes used as a way of "amplifying" plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue. | ||
==Working Concentrations and Stock Solutions== | ==Working Concentrations and Stock Solutions== | ||
High copy plasmids allow 35 μg/ml concentration. Stock solutions can be made at 35 mg/ml in ethanol, kept at -20C. | High copy plasmids allow 35 μg/ml concentration. Low-copy plasmids like [[bacterial artificial chromosomes]] allow 12.5 μg/ml. Stock solutions can be made at 35 mg/ml in ethanol, kept at -20C. | ||
==Resistance Gene== | ==Resistance Gene== | ||
The ''cat'' gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it. | The ''cat'' gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it. | ||
The gene is sometimes specifed as ''Cm<sup>R</sup>'' or ''Cam<sup>R</sup>''. | The gene is sometimes specifed as ''Cm<sup>R</sup>'' or ''Cam<sup>R</sup>''. | ||
==Links== | |||
[http://bacpac.chori.org/clone-handling.htm Children's Hospital Oakland Research Institute (CHORI) - BAC/PAC resources] | |||
[[Category:Material]] [[Category:Antibiotic]] | |||
Latest revision as of 18:52, 12 April 2010
Mode of Action
Chloramphenicol is a bacteriostatic agent that binds to the 50S ribosomal subunit and inhibits ribosomal peptide bond formation. It is sometimes used as a way of "amplifying" plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue.
Working Concentrations and Stock Solutions
High copy plasmids allow 35 μg/ml concentration. Low-copy plasmids like bacterial artificial chromosomes allow 12.5 μg/ml. Stock solutions can be made at 35 mg/ml in ethanol, kept at -20C.
Resistance Gene
The cat gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it. The gene is sometimes specifed as CmR or CamR.
Links
Children's Hospital Oakland Research Institute (CHORI) - BAC/PAC resources
