Chloramphenicol

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==Mode of Action==
==Mode of Action==
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Chloramphenicol is a bacteriostatic agent that binds to the 50S ribosome and inhibits ribosomal peptide bond formation.  It is sometimes uses as a way of "amplifying" plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue.
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Chloramphenicol is a bacteriostatic agent that binds to the 50S ribosomal subunit and inhibits ribosomal peptide bond formation.  It is sometimes used as a way of "amplifying" plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue.
==Working Concentrations and Stock Solutions==
==Working Concentrations and Stock Solutions==

Revision as of 11:16, 13 September 2007

Mode of Action

Chloramphenicol is a bacteriostatic agent that binds to the 50S ribosomal subunit and inhibits ribosomal peptide bond formation. It is sometimes used as a way of "amplifying" plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue.

Working Concentrations and Stock Solutions

High copy plasmids allow 35 μg/ml concentration. Stock solutions can be made at 35 mg/ml in ethanol, kept at -20C.

Resistance Gene

The cat gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it. The gene is sometimes specifed as CmR or CamR.

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