Chloramphenicol

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The ''cat'' gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it.
The ''cat'' gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it.
The gene is sometimes specifed as ''Cm<sup>R</sup>'' or ''Cam<sup>R</sup>''.
The gene is sometimes specifed as ''Cm<sup>R</sup>'' or ''Cam<sup>R</sup>''.
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[[Category:Material]] [[Category:Antibiotic]]

Revision as of 12:45, 26 July 2007

Mode of Action

Chloramphenicol is a bacteriostatic agent that binds to the 50S ribosome and inhibits ribosomal peptide bond formation. It is sometimes uses as a way of "amplifying" plasmid production by shutting down protein synthesis in cultures, while allowing plasmid replication to continue.

Working Concentrations and Stock Solutions

High copy plasmids allow 35 μg/ml concentration. Stock solutions can be made at 35 mg/ml in ethanol, kept at -20C.

Resistance Gene

The cat gene codes for an acetyltransferase which acetylates chloramphenicol, inactivating it. The gene is sometimes specifed as CmR or CamR.

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