Chlamydomonas RNA extraction: Difference between revisions
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Helena Reyes (talk | contribs) (New page: ==Overview== This is a protocol designed to extract RNA from Chlamydomonas reinhardtii. ==Materials== *Chlamydomonas in liquid nutrient medium (take at least one week to grow) *Liquid Ni...) |
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==Overview== | ==Overview== | ||
RNA preparation | |||
This is a protocol designed to extract RNA from Chlamydomonas reinhardtii. | This is a protocol designed to extract RNA from Chlamydomonas reinhardtii. | ||
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*Chloroform (600 μL) | *Chloroform (600 μL) | ||
*NaCl 5M (40 μL) | *NaCl 5M (40 μL) | ||
*Isopropanol ( | *Isopropanol (500 μL) | ||
*Etanol 75% (8 mL) | |||
*Agua mili-q | |||
==Procedure== | |||
Revision as of 09:14, 14 July 2011
Overview
RNA preparation This is a protocol designed to extract RNA from Chlamydomonas reinhardtii.
Materials
- Chlamydomonas in liquid nutrient medium (take at least one week to grow)
- Liquid Nitrogen
- Trizol (500 μL)
- Chloroform (600 μL)
- NaCl 5M (40 μL)
- Isopropanol (500 μL)
- Etanol 75% (8 mL)
- Agua mili-q
