Chang Lab:Notebook/CBE/08/146/2008/12/19

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Entry title

Time start:1pm

Plate count of 18/12/08 plates

  • M9 (1.0 McFarland)
    • 10^-6 : 94
    • 10^-8 : 70
    • 10^-10 : none
  • LB (1.0 McFarland)
    • 10^-10 : too many
    • 10^-12 : 269
    • 10^-14 : 165
    • 10^-16 : 8
    • 10^-18 : 3
    • 10^-20 : none

Prep for bacteria culture

  • Single loop taken from streaked petri dishes and suspended into 10ml broth in a test tube (incubate at 37 C for 24hrs).

Plating at 10^-2, 10^-4 and 10^-6

  • M9 and LB from 21, 30 and 37 C CBDs: too many

Note

  • Redo on 20/12/08