Chang Lab:Notebook/Assessment of Penetration of Biodegradable Nanopolymers into Biofilm/Entry Base
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page |
ProtocolDate: 3 December 2008; Time: 1100; Agenda: Preparation of Ecoli Inoculum Date: 4 December 2008; Time: 0930; Agenda: Quadrant Streaking of Petri Dishes prepared on 3rd December Date: 5 December 2008; Time: 0945; Agenda: Preparation of Ecoli Inoculum and Quadrant Streaking of Petri Dishes Date: 6 December 2008; Time: 0945; Agenda: Store Petri Dishes that were streaked on 5 December in a 4 degrees fridge. Date: 9 December 2008; Time: 0900; Agenda: Mid-Log Phase Determination Date: 13 December 2008; Time: 0915; Agenda: Biofilm formation using M9 and LB Broth. Date: 15 December 2008; Time: 1400; Agenda: Reprepare M9 medium and quantification of biofilm formed Date: 16 December 2008; Time: 0830; Agenda: Biofilm formation at 3 temperatures for 48hr. Date: 17 December 2008; Time: 0830; Agenda: Results of serial plating done on 16 December and sonication done on 15 December. Date: 18 December 2008; Time: 0930; Agenda: Biofilm formation at 3 temperatures for 24hr. Date: 19 December 2008; Time: 0930; Agenda: Quantification of 24hr biofilm at 3 temperatures and results of serial plating done on 18 December. Date: 20 December 2008; Time: 0930; Agenda: Biofilm formation at 3 temperatures for 72hr. Date: 22 December 2008; Time: 0930; Agenda: Culturing of bacteria to grow biofilm the next day. Date: 23 December 2008; Time: 0930; Agenda: Quantification of 72hr biofilm at 3 temperatures and biofilm formation at 21deg and 37deg for 72hr. Date: 26 December 2008; Time: 1400; Agenda: Quantification of 72hr biofilm at 2 temperatures. Date: 27 December 2008; Time: 0930; Agenda: Biofilm formation at 1 temperature for 48hr and 72hr. Date: 29 December 2008; Time: 1400; Agenda: Quantification of 48hr biofilm at 1 temperature. Date: 30 December 2008; Time: 1400; Agenda: Quantification of 72hr biofilm at 1 temperature and biofilm formation at 24hr and 72hr. Date: 31 December 2008; Time: 1400; Agenda: Quantification of 24hr biofilm at 1 temperature. Date: 2 January 2009; Time: 1400; Agenda: Quantification of 72hr biofilm at 1 temperature. Date: 4 January 2009; Time: 1400; Agenda: Streaking of agar plates from slant culture Date: 6 January 2009; Time: 1400; Agenda: Biofilm formation at 1 temperature for 24hr. Date: 7 January 2009; Time: 1400; Agenda: Quantification of 24hr biofilm at 1 temperature and biofilm formation at 1 temperature for 24hr and 48hr. Date: 8 January 2009; Time: 1430; Agenda: Quantification of 24hr and 48hr biofilm at 1 temperature each. Date: 19 January 2009; Time: 1100; Agenda: Culturing of PA01 inoculum. Date: 20 January 2009; Time: 1100; Agenda: Culturing of PA01 inoculum. Date: 21 January 2009; Time: 1100; Agenda: Culturing of PA01 inoculum. Date: 30 January 2009; Time: 1000; Agenda: Mid Log Phase Determination Date: 31 January 2009; Time: 1100; Agenda: Mid Log Phase Determination + Results Date: 2 February 2009; Time:1230; Agenda: Culturing of PA01 inoculum. Date: 3 February 2009; Time:0800; Agenda: Biofilm formation at 21deg and 30deg for 48hr and 72hr. Date: 4 February 2009; Time:1200; Agenda: Culturing of PA01 inoculum. Date: 5 February 2009; Time:0800; Agenda: Biofilm formation at 21deg and 30deg for 24hr + Quantification of 48hr biofilm at 21deg and 30deg. Date: 6 February 2009; Time:0800; Agenda: Culturing of PA01 inoculum (Redo yesterday's 24hr) + Quantification of 72hr biofilm at 21deg and 30deg. Date: 23 February 2009; Time:1400; Agenda: Culturing of Ecoli inoculum for SEM. Date: 24 February 2009; Time:1400; Agenda: Biofilm formation at 37deg, 24hrs for SEM. Date: 25 February 2009; Time:1400; Agenda: Exposure of biofilm to nanopolymers overnight for SEM. Date: 26 February 2009; Time:1400; Agenda: Fixing biofilm and airdrying for SEM. | |
