ChIP-Chip E. coli

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==Abstract==
==Abstract==
ChIP-Chip stands for chromatine immuno precipitation and Chip in the sence of DNA-array. It is a technique to determin the DNA binding sites of a DNA binding protein on a global scale. While the basic priciple is the same there are some differences in handling cells from various organisms. This protocol is developed and tested for '''E. coli'''. It should work the same way for other bacteria but that remains to be proven (please add any experience.)
ChIP-Chip stands for chromatine immuno precipitation and Chip in the sence of DNA-array. It is a technique to determin the DNA binding sites of a DNA binding protein on a global scale. While the basic priciple is the same there are some differences in handling cells from various organisms. This protocol is developed and tested for '''E. coli'''. It should work the same way for other bacteria but that remains to be proven (please add any experience.)
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Other ChIp-Chip protocols can be foun here:
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[Silver: ChIP-chip]
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[Drosophila CHiP on chip]
==Materials==
==Materials==

Revision as of 06:33, 31 March 2008

This protocol is in developement and currently under investigation in the lab. Please contibute and watch changes in the next weeks!

Contents

Curators

Torsten Waldminghaus 06:29, 17 March 2008 (CDT)

Anyone should feel free to add themselves as a curator for this consensus protocol. You do not need to be a curator in order to contribute. The OpenWetWare community is currently discussing the idea of protocol curators. Please contribute.

Abstract

ChIP-Chip stands for chromatine immuno precipitation and Chip in the sence of DNA-array. It is a technique to determin the DNA binding sites of a DNA binding protein on a global scale. While the basic priciple is the same there are some differences in handling cells from various organisms. This protocol is developed and tested for E. coli. It should work the same way for other bacteria but that remains to be proven (please add any experience.)

Other ChIp-Chip protocols can be foun here: [Silver: ChIP-chip] [Drosophila CHiP on chip]

Materials

List everything necessary to perform the protocol here. Include all information about suppliers, ordering details, etc. Links to the suppliers' page on that material are also appropriate and encouraged. Please be aware that users of this protocol may not be working in the same country as you.

Reagents

Biological resources e.g. cell lines, buffers (link to a method for making them), enzymes, chemicals, kits, etc.

Equipment

Any equipment used to perform the protocol (link to a method for using them).

Procedure

A step by step guide to the experimental procedure.


Critical steps

Referenced from the main protocol, a more thorough explanation of particularly important steps in the protocol.

Troubleshooting

Referenced from the main protocol, an explanation of what can cause things to go wrong with the protocol.

Notes

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Anecdotal observations that might be of use to others can also be posted here; e.g. 'my cells were still floating'.

It might also be good to add an image to show the workflow and timescales for experiment planning.

Acknowledgments

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References

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Specific Protocols

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Discussion

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