Cell cycle analysis

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C period = the time for a round of chromosome replication
C period = the time for a round of chromosome replication
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D period = the time between the end of a round of chromosome replication and cell                division
D period = the time between the end of a round of chromosome replication and cell                division
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#Determination of initiation age (a<sub>i</sub>) and C+D:  
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#Determination of initiation age (a<sub>i</sub>) and C+D:[[Image:Theoretical_age_distr.jpg|left|200px]]
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[[Image:Theoretical_age_distr.jpg|left|250px]]
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From flow cytometry analysis of cells treated with rifampicin and cephalexin (run-out histogram) the proportions of cells that had not initiated replication at the time of drug action (4-origin-cells, streaked) and cells that had initiated (8-origin-cells) can be estimated.The initiation age (a<sub>i</sub>) can be found from the theoretical age distribution described by this formula,
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From flow cytometry analysis of cells treated with rifampicin and cephalexin (run-out histogram) the proportions of cells that had notinitiated replication at the time of drug action (4-origin-cells, streaked) and cells that initiated (8-origin-cells) can be estimated.The initiation age (ai) can be found from thetheoretical age distribution described by this formula,
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'''F = 2 - 2<sup>((τ-ai)/τ)</sup>'''
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'''F = 2 - 2<sup>((τ-a<sub>i</sub>)/τ)</sup>'''
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where F is the fraction of cells that had not initiated and is the generation time, or from the estimated graph of the theoretical age distribution (streaked portion).                                                                                                                                                                                     
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where F is the fraction of cells that had not initiated and τ is the generation time, or from the estimated graph of the theoretical age distribution (streaked portion).                                                                                                                                                                                     
                                                                              
                                                                              
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The C+D period is estimated from the initiation age (ai), the generation time () and the number of generations spanned per cell cycle.
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The C+D period is estimated from the initiation age (a<sub>i</sub>), the generation time (τ) and the number of generations spanned per cell cycle.
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Example:

Revision as of 09:59, 15 August 2008

Cell cycle analysis of Escherichia coli cells

C period = the time for a round of chromosome replication

D period = the time between the end of a round of chromosome replication and cell division


  1. Determination of initiation age (ai) and C+D:

From flow cytometry analysis of cells treated with rifampicin and cephalexin (run-out histogram) the proportions of cells that had not initiated replication at the time of drug action (4-origin-cells, streaked) and cells that had initiated (8-origin-cells) can be estimated.The initiation age (ai) can be found from the theoretical age distribution described by this formula,

F = 2 - 2((τ-ai)/τ)

where F is the fraction of cells that had not initiated and τ is the generation time, or from the estimated graph of the theoretical age distribution (streaked portion).




The C+D period is estimated from the initiation age (ai), the generation time (τ) and the number of generations spanned per cell cycle.

Example:

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