Cell cycle analysis: Difference between revisions
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[[Image:Theoretical_age_distr.jpg|left|250px]] | [[Image:Theoretical_age_distr.jpg|left|250px]] | ||
From flow cytometry analysis of cells treated with rifampicin and cephalexin (run-out histogram) the proportions of cells that had notinitiated replication at the time of drug action (4-origin-cells, streaked) and cells that initiated (8-origin-cells) can be estimated.The initiation age (ai) can be found from thetheoretical age distribution described by this formula, | From flow cytometry analysis of cells treated with rifampicin and cephalexin (run-out histogram) the proportions of cells that had notinitiated replication at the time of drug action (4-origin-cells, streaked) and cells that initiated (8-origin-cells) can be estimated.The initiation age (ai) can be found from thetheoretical age distribution described by this formula, | ||
'''F = 2 - 2<sup>((τ-ai)/τ)</sup>''' | |||
where F is the fraction of cells that had not initiated and is the generation time, or from the estimated graph of the theoretical age distribution (streaked portion). | where F is the fraction of cells that had not initiated and is the generation time, or from the estimated graph of the theoretical age distribution (streaked portion). | ||
The C+D period is estimated from the initiation age (ai), the generation time () and the number of generations spanned per cell cycle. | The C+D period is estimated from the initiation age (ai), the generation time () and the number of generations spanned per cell cycle. |
Revision as of 06:43, 15 August 2008
Cell cycle analysis of Escherichia coli cells
C period = the time for a round of chromosome replication D period = the time between the end of a round of chromosome replication and cell division
- Determination of initiation age (ai) and C+D:
From flow cytometry analysis of cells treated with rifampicin and cephalexin (run-out histogram) the proportions of cells that had notinitiated replication at the time of drug action (4-origin-cells, streaked) and cells that initiated (8-origin-cells) can be estimated.The initiation age (ai) can be found from thetheoretical age distribution described by this formula,
F = 2 - 2((τ-ai)/τ)
where F is the fraction of cells that had not initiated and is the generation time, or from the estimated graph of the theoretical age distribution (streaked portion).
The C+D period is estimated from the initiation age (ai), the generation time () and the number of generations spanned per cell cycle.