Cell cycle analysis

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 8: Line 8:
[[Image:Theoretical_age_distr.jpg|left|250px]]
[[Image:Theoretical_age_distr.jpg|left|250px]]
From flow cytometry analysis of cells treated with rifampicin and cephalexin (run-out histogram) the proportions of cells that had notinitiated replication at the time of drug action (4-origin-cells, streaked) and cells that initiated (8-origin-cells) can be estimated.The initiation age (ai) can be found from thetheoretical age distribution described by this formula,
From flow cytometry analysis of cells treated with rifampicin and cephalexin (run-out histogram) the proportions of cells that had notinitiated replication at the time of drug action (4-origin-cells, streaked) and cells that initiated (8-origin-cells) can be estimated.The initiation age (ai) can be found from thetheoretical age distribution described by this formula,
-
*F = 2 - 2<sup>((τ-ai)/τ)</sup>
+
 
 +
'''F = 2 - 2<sup>((τ-ai)/τ)</sup>'''
 +
 
where F is the fraction of cells that had not initiated and  is the generation time, or from the estimated graph of the theoretical age distribution (streaked portion).                                                                                                                                                                                     
where F is the fraction of cells that had not initiated and  is the generation time, or from the estimated graph of the theoretical age distribution (streaked portion).                                                                                                                                                                                     
                                                                              
                                                                              
The C+D period is estimated from the initiation age (ai), the generation time () and the number of generations spanned per cell cycle.
The C+D period is estimated from the initiation age (ai), the generation time () and the number of generations spanned per cell cycle.

Revision as of 09:43, 15 August 2008

Cell cycle analysis of Escherichia coli cells

C period = the time for a round of chromosome replication D period = the time between the end of a round of chromosome replication and cell division


  1. Determination of initiation age (ai) and C+D:

From flow cytometry analysis of cells treated with rifampicin and cephalexin (run-out histogram) the proportions of cells that had notinitiated replication at the time of drug action (4-origin-cells, streaked) and cells that initiated (8-origin-cells) can be estimated.The initiation age (ai) can be found from thetheoretical age distribution described by this formula,

F = 2 - 2((τ-ai)/τ)

where F is the fraction of cells that had not initiated and  is the generation time, or from the estimated graph of the theoretical age distribution (streaked portion).


The C+D period is estimated from the initiation age (ai), the generation time () and the number of generations spanned per cell cycle.

Personal tools