Cell and tissue lysis hub

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This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison.

Contents

Comparison of lysis methods

Sonication

  • most efficient method of cell fractionation
  • problem: heat build up which can denature proteins (proportionate to length of sonication)
  • precaution: do on ice and sonicate intermittantly

Homogenisation

  • best for animal tissue; less suitable for cells
  • precaution: do on ice to reduce heat build-up and denaturation

Freeze-thaw

  • least effective method
  • plus: does not denature proteins as much as other methods

Detergents

  • chemical method of lysis
  • problems: detergent may inhibit subsequent reactions
  • problems: detergent may disrupts protein interactions

Specific protocols

description/link target lysis method type of material
Silver: Lysate for Western protein detergent (Triton) eukaryotic cells
Sauer:Lysing E. coli with Lysozymes lysozyme bacteria
Blackburn:Yeast Colony PCR DNA NaOH yeast
Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer protein detergent (RIPA buffer) cell line
Streptomyces:Protocols/Mini-Maxi Prep DNA NaOH bacteria
Western Blot/Tissue Preparation protein detergent & physical disruption tissue
add another method's name target lysis method type of starting material
add another method's name target lysis method type of starting material
add another method's name target lysis method type of starting material

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