Cell and tissue lysis hub

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[[Image:Hub icon.png|right|thumbnail|protocol hub]]
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[[Image:Hub icon.png|right|thumbnail|'''protocol hub''']]
This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison.
This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison.
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| [[Eccles:Protein Lysates from Cells in Culture]]
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| target
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| protein
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| lysis method
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| detergent (SDS, NP40)
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| type of starting material
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| cell line
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| [[Eccles:Protein Lysates from Tissue]]
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| target
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| protein
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| lysis method
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| detergent, physical, freezing
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| type of starting material
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| tissue
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| [[RNA extraction using trizol/tri]]
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| RNA
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| phenol
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| cell line
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| add another method's name
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== Related hub pages ==
== Related hub pages ==

Revision as of 07:07, 26 June 2008

protocol hub
protocol hub

This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison.

Contents

Comparison of lysis methods

Sonication

  • most efficient method of cell fractionation
  • problem: heat build up which can denature proteins (proportionate to length of sonication)
  • precaution: do on ice and sonicate intermittantly

Homogenisation

  • best for animal tissue; less suitable for cells
  • precaution: do on ice to reduce heat build-up and denaturation

Freeze-thaw

  • least effective method
  • plus: does not denature proteins as much as other methods

Detergents

  • chemical method of lysis
  • problems: detergent may inhibit subsequent reactions
  • problems: detergent may disrupts protein interactions

Specific protocols

description/link target lysis method type of material
Silver: Lysate for Western protein detergent (Triton) eukaryotic cells
Sauer:Lysing E. coli with Lysozymes lysozyme bacteria
Blackburn:Yeast Colony PCR DNA NaOH yeast
Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer protein detergent (RIPA buffer) cell line
Streptomyces:Protocols/Mini-Maxi Prep DNA NaOH bacteria
Western Blot/Tissue Preparation protein detergent & physical disruption tissue
Eccles:Protein Lysates from Cells in Culture protein detergent (SDS, NP40) cell line
Eccles:Protein Lysates from Tissue protein detergent, physical, freezing tissue
RNA extraction using trizol/tri RNA phenol cell line
add another method's name target lysis method type of starting material


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