Cell and tissue lysis hub

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== Specific protocols ==
== Specific protocols ==
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* [[Silver: Lysate for Western]] - Silver Lab: cell lysis for [[Western]] (method: Triton)
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* [[Sauer:Lysing E. coli with Lysozymes]] - Sauer Lab: bacteria lysis (method: lysozyme)
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{| {{table}}
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* [[Blackburn:Yeast Colony PCR]] - Blackburn Lab: yeast lysis for [[colony PCR]] (method NaOH)
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|+ '''caption'''
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* [[Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer]] - Jacobs Lab: cell line lysis for protein (method: RIPA buffer)
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|-
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* [[Streptomyces:Protocols/Mini-Maxi Prep]] - Streptomyces community, East Anglia: bacteria lysis for DNA (method: NaOH)
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! style="background:lightgrey"|description/link
 +
! style="background:lightgrey"|target
 +
! style="background:lightgrey"|lysis method
 +
! style="background:lightgrey"|type of material
 +
|-
 +
| [[Silver: Lysate for Western]]
 +
| protein
 +
| detergent (Triton)
 +
| eukaryotic cells
 +
|-
 +
| [[Sauer:Lysing E. coli with Lysozymes]]
 +
|
 +
| lysozyme
 +
| bacteria
 +
|-
 +
| [[Blackburn:Yeast Colony PCR]]
 +
| DNA
 +
| NaOH
 +
| yeast
 +
|-
 +
| [[Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer]]
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| protein
 +
| detergent (RIPA buffer)
 +
| cell line
 +
|-
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| [[Streptomyces:Protocols/Mini-Maxi Prep]]
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| DAN
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| NaOH
 +
| bacteria
 +
|-
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| add another method's name
 +
| target
 +
| lysis method
 +
| type of starting material
 +
|}
== Related discussions on BioForum ==
== Related discussions on BioForum ==

Revision as of 06:44, 26 June 2008

This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison.

Contents

Comparison of lysis methods

Sonication

  • most efficient method of cell fractionation
  • problem: heat build up which can denature proteins (proportionate to length of sonication)
  • precaution: do on ice and sonicate intermittantly

Homogenisation

  • best for animal tissue; less suitable for cells
  • precaution: do on ice to reduce heat build-up and denaturation

Freeze-thaw

  • least effective method
  • plus: does not denature proteins as much as other methods

Detergents

  • chemical method of lysis
  • problems: detergent may inhibit subsequent reactions
  • problems: detergent may disrupts protein interactions

Specific protocols

caption
description/link target lysis method type of material
Silver: Lysate for Western protein detergent (Triton) eukaryotic cells
Sauer:Lysing E. coli with Lysozymes lysozyme bacteria
Blackburn:Yeast Colony PCR DNA NaOH yeast
Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer protein detergent (RIPA buffer) cell line
Streptomyces:Protocols/Mini-Maxi Prep DAN NaOH bacteria
add another method's name target lysis method type of starting material

Related discussions on BioForum

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