Cell and tissue lysis hub

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* [[Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer]] - Jacobs Lab: cell line lysis for protein (method: RIPA buffer)
* [[Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer]] - Jacobs Lab: cell line lysis for protein (method: RIPA buffer)
* [[Streptomyces:Protocols/Mini-Maxi Prep]] - Streptomyces community, East Anglia: bacteria lysis for DNA (method: NaOH)
* [[Streptomyces:Protocols/Mini-Maxi Prep]] - Streptomyces community, East Anglia: bacteria lysis for DNA (method: NaOH)
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== Related discussions on BioForum ==
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* [http://www.protocol-online.org/forums/index.php?showtopic=23747 cell lysis methods comparison]
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* [http://www.protocol-online.org/forums/index.php?showtopic=1943&hl=cell+lysis what solution to use for cell lysis]

Revision as of 05:26, 26 June 2008

This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison.

Contents

Comparison of lysis methods

Sonication

  • most efficient method of cell fractionation
  • problem: heat build up which can denature proteins (proportionate to length of sonication)
  • precaution: do on ice and sonicate intermittantly

Homogenisation

  • best for animal tissue; less suitable for cells
  • precaution: do on ice to reduce heat build-up and denaturation

Freeze-thaw

  • least effective method
  • plus: does not denature proteins as much as other methods

Detergents

  • chemical method of lysis
  • problems: detergent may inhibit subsequent reactions
  • problems: detergent may disrupts protein interactions

Specific protocols

Related discussions on BioForum

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