Cell and tissue lysis hub

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(comparison of lysis method section)
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This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison.
This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison.
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* [[Silver: Lysate for Western]] - Silver Lab: cell lysis for [[Western]] (method: Triton)
 
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* [[Sauer:Lysing E. coli with Lysozymes]] - Sauer Lab: bacteria lysis (method: lysozyme)
 
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* [[Blackburn:Yeast Colony PCR]] - Blackburn Lab: yeast lysis for [[colony PCR]] (method NaOH)
 
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* [[Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer]] - Jacobs Lab: cell line lysis for protein (method: RIPA buffer)
 
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* [[Streptomyces:Protocols/Mini-Maxi Prep]] - Streptomyces community, East Anglia: bacteria lysis for DNA (method: NaOH)
 
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== Comparison of lysis methods ==
== Comparison of lysis methods ==
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* problems: detergent may inhibit subsequent reactions
* problems: detergent may inhibit subsequent reactions
* problems: detergent may disrupts protein interactions
* problems: detergent may disrupts protein interactions
 +
 +
== Specific protocols ==
 +
* [[Silver: Lysate for Western]] - Silver Lab: cell lysis for [[Western]] (method: Triton)
 +
* [[Sauer:Lysing E. coli with Lysozymes]] - Sauer Lab: bacteria lysis (method: lysozyme)
 +
* [[Blackburn:Yeast Colony PCR]] - Blackburn Lab: yeast lysis for [[colony PCR]] (method NaOH)
 +
* [[Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer]] - Jacobs Lab: cell line lysis for protein (method: RIPA buffer)
 +
* [[Streptomyces:Protocols/Mini-Maxi Prep]] - Streptomyces community, East Anglia: bacteria lysis for DNA (method: NaOH)

Revision as of 05:17, 26 June 2008

This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison.

Contents

Comparison of lysis methods

Sonication

  • most efficient method of cell fractionation
  • problem: heat build up which can denature proteins (proportionate to length of sonication)
  • precaution: do on ice and sonicate intermittantly

Homogenisation

  • best for animal tissue; less suitable for cells
  • precaution: do on ice to reduce heat build-up and denaturation

Freeze-thaw

  • least effective method
  • plus: does not denature proteins as much as other methods

Detergents

  • chemical method of lysis
  • problems: detergent may inhibit subsequent reactions
  • problems: detergent may disrupts protein interactions

Specific protocols

Personal tools