Cconboy:Terminator Characterization/Results: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 2: Line 2:


[[Image:Cconboy Terminator Characterization Normalized Flow Data.jpg|center]]
[[Image:Cconboy Terminator Characterization Normalized Flow Data.jpg|center]]
Fig. 1: Normalized mean CFP and YFP fluorescence per cell of terminator characterization constructs. Mean fluorescence per cell was measured by flow cytometry. Values were normalized to I7003, the 0% termination efficiency control construct.
Fig. 1: Normalized mean CFP and YFP fluorescence per cell of terminator characterization constructs. Mean fluorescence per cell was measured by flow cytometry. Values were normalized to I7003, the 0% termination efficiency control construct. Raw data available here [[cconboy:Terminator Characterization/sup mat|here]]. 


{| border = "1" cellpadding="5" cellspacing="0" align="left"
{| border = "1" cellpadding="5" cellspacing="0" align="left"
|+ '''Table 1: Terminator Characterization Flow Data'''
|+ '''Table 1: Terminator Characterization Flow Data'''
|-
|-
! Construct !! Terminator !! Mean YFP (au) !! Mean CFP (au) !! Termination Efficiency
! Forward Terminator !! Average Forward Termination Efficiency !! Average Reverse Termination Efficiency !! Reverse Terminator
|-
|-
| -- || Negative Control: DH5a only || 53 || 53|| n/a ||
| B0011 || 41.9 % || 63.6 % || B0021 ||
|-
|-
| BBa_I7000 || Positive Control: CFP only || 140 || 7149 || n/a ||
| B0012 || 30.9 % || -36.8 % || B0022 ||
|-
|-
| BBa_I7001 || Positive Control: YFP only || 13548 || 39 || n/a ||
| B0013 || 60.0 % || -106.0 % || B0023 ||
|-
|-
| BBa_I7003 || Postitive Control: CFP.YFP || 5483 || 1852 || 0.0 % ||
| B0014 || 60.4 % || * || B0024 ||
|-
|-
| BBa_I7011 || B0011 || 4569 || 2658 || 41.9 % ||
| B0015 || 98.4 % || 29.5 % || B0025 ||
|-
|-
| BBa_I7012 || B0012 || 7821 || 3823 || 30.9 % ||
|-
| BBa_I7013 || B0013 || 5199 || 4388 || 60.0 % ||
|-
| BBa_I7014 || B0014 || 3975 || 3390 || 60.4 % ||
|-
| BBa_I7015 || B0015 || 324 || 6777 || 98.4 % ||
|-
| BBa_I7021 || B0021 || 3906 || 3627 || 63.6 % ||
|-
| BBa_I7022 || B0022 || 5312 || 1312 || -36.8 % ||
|-
| BBa_I7023 || B0023 || 9574 || 1570 || -106.0 % ||
|-
| BBa_I7025 || B0025 || 6945 || 3328 || 29.5 % ||
|-
===

Revision as of 11:58, 6 April 2006

This data is being reanalyzed to consider single-cell termination efficiencies rather than the population average. See terminator characterization work by jason kelly for an analysis protocol. Will also update to add raw flow data at that time.

Fig. 1: Normalized mean CFP and YFP fluorescence per cell of terminator characterization constructs. Mean fluorescence per cell was measured by flow cytometry. Values were normalized to I7003, the 0% termination efficiency control construct. Raw data available here here.

Table 1: Terminator Characterization Flow Data
Forward Terminator Average Forward Termination Efficiency Average Reverse Termination Efficiency Reverse Terminator
B0011 41.9 % 63.6 % B0021
B0012 30.9 % -36.8 % B0022
B0013 60.0 % -106.0 % B0023
B0014 60.4 % * B0024
B0015 98.4 % 29.5 % B0025
Retrieved from "https://openwetware.org/mediawiki/index.php?title=Cconboy:Terminator_Characterization/Results&oldid=29212"