Cconboy:Terminator Characterization/Results: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
mNo edit summary |
mNo edit summary |
||
| Line 4: | Line 4: | ||
|+ '''Table 1: Terminator Characterization Flow Data''' | |+ '''Table 1: Terminator Characterization Flow Data''' | ||
|- | |- | ||
! Construct !! Terminator !! Mean YFP !! Mean CFP !! Termination Efficiency | ! Construct !! Terminator !! Mean YFP (au) !! Mean CFP (au) !! Termination Efficiency | ||
|- | |- | ||
| -- || Negative Control: DH5a only || 53 || 53|| n/a || | | -- || Negative Control: DH5a only || 53 || 53|| n/a || | ||
Revision as of 15:16, 23 March 2006
This data is being reanalyzed to consider single-cell termination efficiencies rather than the population average. See terminator characterization work by jason kelly for an analysis protocol. Will also update to add raw flow data at that time.
=
| Construct | Terminator | Mean YFP (au) | Mean CFP (au) | Termination Efficiency | |
|---|---|---|---|---|---|
| -- | Negative Control: DH5a only | 53 | 53 | n/a | |
| BBa_I7000 | Positive Control: CFP only | 140 | 7149 | n/a | |
| BBa_I7001 | Positive Control: YFP only | 13548 | 39 | n/a | |
| BBa_I7003 | Postitive Control: CFP.YFP | 5483 | 1852 | 0.0 % | |
| BBa_I7011 | B0011 | 4569 | 2658 | 41.9 % | |
| BBa_I7012 | B0012 | 7821 | 3823 | 30.9 % | |
| BBa_I7013 | B0013 | 5199 | 4388 | 60.0 % | |
| BBa_I7014 | B0014 | 3975 | 3390 | 60.4 % | |
| BBa_I7015 | B0015 | 324 | 6777 | 98.4 % | |
| BBa_I7021 | B0021 | 3906 | 3627 | 63.6 % | |
| BBa_I7022 | B0022 | 5312 | 1312 | -36.8 % | |
| BBa_I7023 | B0023 | 9574 | 1570 | -106.0 % | |
| BBa_I7025 | B0025 | 6945 | 3328 | 29.5 % |
