Cconboy:Terminator Characterization: Difference between revisions
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===Background=== | ===Background=== | ||
The rational design of integrated biological systems requires a set of characterized, compatible components. Here we characterize | The rational design of integrated biological systems requires a set of characterized, compatible components. Here we characterize a set of transcriptional terminators contained in the registry of standard biological parts. [http://parts.mit.edu/r/parts/partsdb/pgroup.cgi?pgroup=Terminator]. We measure termination efficiency by inserting each terminator between the coding regions for CFP and YFP on a polycistronic message and measuring the fluorescence ratio of the two fluorophores by flow cytometry. | ||
[[cconboy:Terminator Characterization/Constructs|Constructs]] | [[cconboy:Terminator Characterization/Constructs|Constructs]] | ||
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===Related=== | ===Related=== | ||
[[PoPS device screening plasmid]] | [[PoPS device screening plasmid]] | ||
* Subsequent terminator characterization work by Jason Kelly, Josh Michener and Kelly Chang | * Subsequent terminator characterization work by Jason Kelly, Josh Michener and Kelly Chang. | ||
[http://parts.mit.edu/r/parts/partsdb/pgroup.cgi?pgroup=Terminator Transcriptional Terminators in the Registry] | [http://parts.mit.edu/r/parts/partsdb/pgroup.cgi?pgroup=Terminator Transcriptional Terminators in the Registry] | ||
Revision as of 13:01, 23 March 2006
Page in Progress
Experiments performed by Caitlin Conboy, Fall 2003
Background
The rational design of integrated biological systems requires a set of characterized, compatible components. Here we characterize a set of transcriptional terminators contained in the registry of standard biological parts. [1]. We measure termination efficiency by inserting each terminator between the coding regions for CFP and YFP on a polycistronic message and measuring the fluorescence ratio of the two fluorophores by flow cytometry.
Related
- Subsequent terminator characterization work by Jason Kelly, Josh Michener and Kelly Chang.
