Cconboy:Promoter Characterization/Constructs
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Constructs in this study were assembled from parts in the Regisry via standard assembly. All constructs were cloned onto pSB1A2, a high copy plasmid, in MC4100.
Fig.1: Schematic of Promoter Characterization Constructs
| Construct | Promoter | Description | |
|---|---|---|---|
| BBa_I6030 | R0010 | LacI regulated | |
| BBa_I6031 | R0011 | Hybrid promoter based on lambda pL, repressed by LacI | |
| BBa_I6032 | R0040 | Hybrid promoter based on cI, repressed by TetR | |
| BBa_I6034 | R0050 | HK022 cI regulated | |
| BBa_I6036 | R0051 | Lambda cI regulated | |
| BBa_I6038 | R0052 | 434 cI regulated | |
| BBa_I6040 | R0053 | p22 cII regulated | |
| BBa_I6042 | R0062 | lux pR from V.fischeri, activated by HSL-bound LuxR | |
| BBa_I6044 | R0063 | lux pL from V.fischeri, repressed by HSL-bound LuxR | |
| BBa_I6046 | R0065 | Hybrid promoter, repressed by lambda cI, activated by HSL-bound LuxR | |
| BBa_I6048 | R1050 | Standard promoter, repressed by HK022 cI | |
| BBa_I6050 | R1051 | Standard promoter, repressed by lambda cI | |
| BBa_I6052 | R1052 | Standard promoter, repressed by 434 cI | |
| BBa_I6054 | R1053 | Standard promoter, repressed by p22 cII | |
| BBa_I6056 | R1062 | Standard promoter, activated by HSL-bound LuxR | |
| BBa_I6058 | I0500 | pBad/AraC, arabinose-inducible promoter |
