Category:Construction of Optical Tweezers and devices - Revision history

Pranav Rathi: /* Fiber light for Olympus microscope */

Pranav Rathi — Mon, 28 Jan 2013 17:42:19 GMT

Fiber light for Olympus microscope

←Older revision		Revision as of 17:42, 28 January 2013
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	===Fiber light for Olympus microscope===		===Fiber light for Olympus microscope===
-	Optical tweezers is enclosed due to extreme sensitivity to airborne noise and current. Microscope (with halogen lamp illuminator) enclosed is subjected to temperature hikes inside enclosure ~~and~~ not good for DNA-experiments. To solve the problem I invented a fiber-light system for Olympus 1X71 microscope. The post discusses the make [http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2012/09/21/Fiber_light_for_Olympus_microscope].	+	Optical tweezers is enclosed due to extreme sensitivity to airborne noise and current. Microscope (with halogen lamp illuminator) enclosed is subjected to temperature hikes inside enclosure which is not good for DNA-experiments. To solve the problem I invented a fiber-light system for Olympus 1X71 microscope. The post discusses the make [http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2012/09/21/Fiber_light_for_Olympus_microscope].

	https://lh4.googleusercontent.com/-6tY6sZyHr1c/UQBng8pUgcI/AAAAAAAACFk/VWkBvewiN_c/s144/DSC_0453.jpg		https://lh4.googleusercontent.com/-6tY6sZyHr1c/UQBng8pUgcI/AAAAAAAACFk/VWkBvewiN_c/s144/DSC_0453.jpg

Pranav Rathi: /* Easy way to align two laser beams */

Pranav Rathi — Sat, 26 Jan 2013 22:47:34 GMT

Easy way to align two laser beams

←Older revision		Revision as of 22:47, 26 January 2013
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	===Easy way to align two laser beams===		===Easy way to align two laser beams===
-	In OT I use 1064nm IR laser for trapping, since it is invisible I align it with 633nm HeNe which helps me in ~~number of~~ ways: It let me align IR in farfield especially when i need to align the microscope and ~~with HeNe~~ it ~~is easier to~~ find the sample plane in eyepiece ~~and~~ camera. This post discusses the procedure-steps of alignment[http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2010/04/20/Super_easy_way_to_straighten_and_align_two_laser_beams_simultaneously]	+	In OT I use 1064nm IR laser for trapping, since it is invisible I align it with 633nm HeNe which helps me in many ways: It let me align IR in farfield especially when i need to align the microscope and it helps me find the sample plane in eyepiece with camera. This post discusses the procedure-steps of alignment[http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2010/04/20/Super_easy_way_to_straighten_and_align_two_laser_beams_simultaneously]

	https://lh3.googleusercontent.com/-hoO2OWB5riQ/UP9A4vopHhI/AAAAAAAACC8/mGBc7KlrABQ/s144/TEATA.png		https://lh3.googleusercontent.com/-hoO2OWB5riQ/UP9A4vopHhI/AAAAAAAACC8/mGBc7KlrABQ/s144/TEATA.png

Pranav Rathi: /* Opto-mechanical controls with Lego */

Pranav Rathi — Fri, 25 Jan 2013 22:22:55 GMT

Opto-mechanical controls with Lego

←Older revision		Revision as of 22:22, 25 January 2013
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	https://lh4.googleusercontent.com/-78sQOwI4NJU/UP9RrU0rgZI/AAAAAAAACFE/Iof8I3IW-Xw/s144/Microscope%2520enclosure%2520first%2520steering-lens%2520arrangment2.jpg		https://lh4.googleusercontent.com/-78sQOwI4NJU/UP9RrU0rgZI/AAAAAAAACFE/Iof8I3IW-Xw/s144/Microscope%2520enclosure%2520first%2520steering-lens%2520arrangment2.jpg
		+
		+	===Fiber light for Olympus microscope===
		+	Optical tweezers is enclosed due to extreme sensitivity to airborne noise and current. Microscope (with halogen lamp illuminator) enclosed is subjected to temperature hikes inside enclosure and not good for DNA-experiments. To solve the problem I invented a fiber-light system for Olympus 1X71 microscope. The post discusses the make [http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2012/09/21/Fiber_light_for_Olympus_microscope].
		+
		+	https://lh4.googleusercontent.com/-6tY6sZyHr1c/UQBng8pUgcI/AAAAAAAACFk/VWkBvewiN_c/s144/DSC_0453.jpg

Pranav Rathi: /* Opto-mechanical controls with Lego */

Pranav Rathi — Wed, 23 Jan 2013 17:40:26 GMT

Opto-mechanical controls with Lego

←Older revision		Revision as of 17:40, 23 January 2013
Line 44:		Line 44:
	Optical trap is really sensitive to airborne noise and currents. To deal with the problem I put an enclosure around the microscope. Everything enclosed makes it hard to adjust things inside. With 3 service doors everything is reachable inside the enclosure but it's still hard if trap and QPD position has to be adjusted before every data acquisition. So to make my life easier I extended the opto-mechanical controls on XY translator for steering (trap position) lens and QPD. The post discusses the make [http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2012/01/12/Extend_your_Opto-mechanical_controls_with_Lego_for_out_of_box_control]		Optical trap is really sensitive to airborne noise and currents. To deal with the problem I put an enclosure around the microscope. Everything enclosed makes it hard to adjust things inside. With 3 service doors everything is reachable inside the enclosure but it's still hard if trap and QPD position has to be adjusted before every data acquisition. So to make my life easier I extended the opto-mechanical controls on XY translator for steering (trap position) lens and QPD. The post discusses the make [http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2012/01/12/Extend_your_Opto-mechanical_controls_with_Lego_for_out_of_box_control]

-	~~http~~://~~openwetware~~.~~org~~/~~wiki~~/~~User:Pranav_Rathi~~/~~Notebook~~/OT/~~2012~~/~~01/12/Extend_your_Opto~~-~~mechanical_controls_with_Lego_for_out_of_box_control~~	+	https://lh4.googleusercontent.com/-78sQOwI4NJU/UP9RrU0rgZI/AAAAAAAACFE/Iof8I3IW-Xw/s144/Microscope%2520enclosure%2520first%2520steering-lens%2520arrangment2.jpg

Pranav Rathi: /* Opto-mechanical controls with Lego */

Pranav Rathi — Wed, 23 Jan 2013 17:39:13 GMT

Opto-mechanical controls with Lego

←Older revision		Revision as of 17:39, 23 January 2013
Line 42:		Line 42:

	===Opto-mechanical controls with Lego===		===Opto-mechanical controls with Lego===
-	Optical trap is really sensitive to airborne noise and currents. To deal with the problem I put an enclosure around the microscope. Everything enclosed makes	+	Optical trap is really sensitive to airborne noise and currents. To deal with the problem I put an enclosure around the microscope. Everything enclosed makes it hard to adjust things inside. With 3 service doors everything is reachable inside the enclosure but it's still hard if trap and QPD position has to be adjusted before every data acquisition. So to make my life easier I extended the opto-mechanical controls on XY translator for steering (trap position) lens and QPD. The post discusses the make [http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2012/01/12/Extend_your_Opto-mechanical_controls_with_Lego_for_out_of_box_control]
		+
		+	http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2012/01/12/Extend_your_Opto-mechanical_controls_with_Lego_for_out_of_box_control

Pranav Rathi: /* Opto-mechanical controls with Lego */

Pranav Rathi — Wed, 23 Jan 2013 16:54:31 GMT

Opto-mechanical controls with Lego

←Older revision		Revision as of 16:54, 23 January 2013
Line 42:		Line 42:

	===Opto-mechanical controls with Lego===		===Opto-mechanical controls with Lego===
-	Optical trap is really sensitive to airborne noise and ~~air-~~currents.	+	Optical trap is really sensitive to airborne noise and currents. To deal with the problem I put an enclosure around the microscope. Everything enclosed makes

Pranav Rathi: /* Fast laser shutter */

Pranav Rathi — Wed, 23 Jan 2013 16:52:02 GMT

Fast laser shutter

←Older revision		Revision as of 16:52, 23 January 2013
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	===Fast laser shutter===		===Fast laser shutter===
-	During DNA experiments I need to center the trap over the tethered bead very precisely, to get the geometry right (this affects the force measurement) and make our feedback program run. To center the tether I need to turn the laser intensity (trap) on/off quickly for various time intervals. To do this I used to use AOM , because it’s extremely quick (nano second on/off time). But AOM has some inherent oscillation problem which makes its use as a switch unsuccessful. So I needed something else, (a shutter) which can replace this AOM function. So I invented the fast shutter, which does it exactly with an opening time of 4 and closing time of 2 μsec. The shutter runs on a +5 volts and there is hardly any electronics involved. It is controlled through a foot-switch. The post discusses the make [http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2011/03/01/Laser_Shutter_.2]	+	During DNA experiments I need to center the trap over the tethered bead very precisely, to get the geometry right (this affects the force measurement) and make our feedback program run. To center the tether I need to turn the laser intensity (trap) on/off quickly for various time intervals. To do this I used to use AOM , because it’s extremely quick (nano second on/off time). But AOM has some inherent oscillation problem which makes its use as a switch unsuccessful. So I needed something else (a shutter) which can replace this AOM function. So I invented the fast shutter, which does it exactly with an opening time of 4 and closing time of 2 μsec. The shutter runs on a +5 volts and there is hardly any electronics involved. It is controlled through a foot-switch. The post discusses the make [http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2011/03/01/Laser_Shutter_.2]

	https://lh5.googleusercontent.com/-1sCu8D4orHc/UP9GGC0JiAI/AAAAAAAACDs/eEJ36P0sAKA/s144/800px-Shutter1.JPG		https://lh5.googleusercontent.com/-1sCu8D4orHc/UP9GGC0JiAI/AAAAAAAACDs/eEJ36P0sAKA/s144/800px-Shutter1.JPG

Pranav Rathi: /* Fast laser shutter */

Pranav Rathi — Wed, 23 Jan 2013 16:50:51 GMT

Fast laser shutter

←Older revision		Revision as of 16:50, 23 January 2013
Line 22:		Line 22:

	===Fast laser shutter===		===Fast laser shutter===
-	During DNA experiments I need to center the trap over the tethered bead very precisely, to get the geometry right (this affects the force measurement) and make our feedback program run. To center the tether I need to turn the laser intensity (trap) on/off quickly for various time intervals. To do this I used to use AOM , because it’s extremely quick (nano second on/off time). But AOM has some inherent oscillation problem which makes it use as a switch, unsuccessful. So I needed something else, (a shutter) which can replace this AOM function. So I invented the fast shutter, which does it exactly with an opening time of 4 and closing time of 2 μsec. The shutter runs on a +5 volts and there is hardly any electronics involved. It is controlled through a foot-switch. The post discusses the make [http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2011/03/01/Laser_Shutter_.2]	+	During DNA experiments I need to center the trap over the tethered bead very precisely, to get the geometry right (this affects the force measurement) and make our feedback program run. To center the tether I need to turn the laser intensity (trap) on/off quickly for various time intervals. To do this I used to use AOM , because it’s extremely quick (nano second on/off time). But AOM has some inherent oscillation problem which makes its use as a switch unsuccessful. So I needed something else, (a shutter) which can replace this AOM function. So I invented the fast shutter, which does it exactly with an opening time of 4 and closing time of 2 μsec. The shutter runs on a +5 volts and there is hardly any electronics involved. It is controlled through a foot-switch. The post discusses the make [http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2011/03/01/Laser_Shutter_.2]

	https://lh5.googleusercontent.com/-1sCu8D4orHc/UP9GGC0JiAI/AAAAAAAACDs/eEJ36P0sAKA/s144/800px-Shutter1.JPG		https://lh5.googleusercontent.com/-1sCu8D4orHc/UP9GGC0JiAI/AAAAAAAACDs/eEJ36P0sAKA/s144/800px-Shutter1.JPG

Pranav Rathi: /* Acoustic and mechanical noise reader */

Pranav Rathi — Wed, 23 Jan 2013 16:45:03 GMT

Acoustic and mechanical noise reader

←Older revision		Revision as of 16:45, 23 January 2013
Line 40:		Line 40:

	https://lh3.googleusercontent.com/-UzMIDjLfHfE/UP9NCdjCtXI/AAAAAAAACEk/dDTa7WWzwRk/s144/AMNR.png		https://lh3.googleusercontent.com/-UzMIDjLfHfE/UP9NCdjCtXI/AAAAAAAACEk/dDTa7WWzwRk/s144/AMNR.png
		+
		+	===Opto-mechanical controls with Lego===
		+	Optical trap is really sensitive to airborne noise and air-currents.

Pranav Rathi: /* X-Y stage control with joystick */

Pranav Rathi — Wed, 23 Jan 2013 16:42:18 GMT

X-Y stage control with joystick

←Older revision		Revision as of 16:42, 23 January 2013
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	https://lh3.googleusercontent.com/-Ls6Yeqk_fVo/UP9JupIlpwI/AAAAAAAACEQ/6BXLYCpUtyg/s144/X-Y%2520stage%2520control%2520with%2520joystick.png		https://lh3.googleusercontent.com/-Ls6Yeqk_fVo/UP9JupIlpwI/AAAAAAAACEQ/6BXLYCpUtyg/s144/X-Y%2520stage%2520control%2520with%2520joystick.png
		+
		+	===Acoustic and mechanical noise reader===
		+	Optical trap is really sensitive to airborne and mechanical vibrations in setup. I was receiving both in my data. So I needed something to study the noise and identify its sources. One way to do it to use piezo-sensor which is not easy to use. I needed some thing easier and quicker. So i invented AMNR out of class-2 stethoscope and a sensitive condenser mic. Condenser mic let me listen to airborne noise and stethoscope let me listen to mechanical vibrations. I use computer sound-card to acquire data and use LabVIEW to process it. The post discusses the make.[http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2011/10/11/Device_for_studying_acoustic_and_mechanical_noise_in_optical_setups]
		+
		+	https://lh3.googleusercontent.com/-UzMIDjLfHfE/UP9NCdjCtXI/AAAAAAAACEk/dDTa7WWzwRk/s144/AMNR.png