Carmen E. Castaneda: Week 9

Make a list of at least 10 biological terms for which you did not know the definitions when you first read the article. Define each of the terms. You can use the glossary in any molecular biology, cell biology, or genetics text book as a source for definitions, or you can use one of many available online biological dictionaries (links below). List the citation(s) for the dictionary(s) you use, providing a URL to the page is fine.

1. trehalose (1)
2. osmolarity (2)
3. dendrogram (3)
4. ribosome biogenesis (4)
5. glycogen (5)
6. diauxic (6)

1. Write an outline of the article. The length should be the equivalent of 2 pages of standard 8 1/2 by 11 inch paper (you can check this by clicking on "Print preview" in your browser). Your outline can be in any form you choose, but you should utilize the wiki syntax of headers and either numbered or bulleted lists to create it. The text of the outline does not have to be complete sentences, but it should answer the questions listed below and have enough information so that others can follow it. However, your outline should be in YOUR OWN WORDS, not copied straight from the article.
   • What is the main result presented in this paper?
   • What is the importance or significance of this work?
   • How did they treat the cells (what experiment were they doing?)
   • What strain(s) of yeast did they use? Was the strain haploid or diploid?
   • What media did they grow them in? What temperature? What type of incubator? For how long?
   • What controls did they use?
   • How many replicates did they perform per timepoint?
   • What mathematical/statistical method did they use to analyze the data?
   • What transcription factors did they talk about?
   • Briefly state the result shown in each of the figures and tables.

Outline

Abstract

• transcriptional response of budding yeast to cold
  • exposed to 10°C for different lengths of time
  • DNA microarrays were used characterize the changes in transcript abundance
    • two main groups were identified, early cold response, ERC, and late cold response, LRC
  • overlap between environmental stress response, ESR, genes and LRC
  • acoomulation of the carbohydrate reserve trehalose and glycogen is indunced during LRC
  • strand munipulation
    • Msn2p and Msn4p involvement with the inductionof genes common to stress responses
      • recognition of paterns during LRC and comparirison between ERC

Introduction

1. Each group of students will be assigned one section of the paper. The group will be responsible for preparing that portion of the PowerPoint presentation and explaining the assigned section and figures in detail to the class. Groups will be assigned on 3/10/11 in class.
   • Introduction, Figures 1, 2: Sarah, Carmen
   • Methods, Figures 3, 4: Alondra, Nick
   • Discussion, Figures 5, 6: James