Carbenicillin - Revision history

Christopher C Vanlang: /* Plasmid stabilization */

Christopher C Vanlang — Thu, 05 Jan 2012 06:44:35 GMT

Plasmid stabilization

←Older revision		Revision as of 06:44, 5 January 2012
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	==Plasmid stabilization==		==Plasmid stabilization==
-	**Carbenicillin is used to select for cells bearing the beta-lactamase gene ''bla'' or ''ampR''. Beta-lactamase also neutralizes ampicillin, but due to ampicillin's lower effectiveness, carbenicillin tends to give fewer satellite colonies and makes for more reliable cloning. Ampicillin is more widely used, though, due to carbenicillin's high cost ($75/g compared to ampicillin's $10/g (Sigma)).	+	*Carbenicillin is used to select for cells bearing the beta-lactamase gene ''bla'' or ''ampR''. Beta-lactamase also neutralizes ampicillin, but due to ampicillin's lower effectiveness, carbenicillin tends to give fewer satellite colonies and makes for more reliable cloning. Ampicillin is more widely used, though, due to carbenicillin's high cost ($75/g compared to ampicillin's $10/g (Sigma)).

	[[Category:Material]] [[Category:Antibiotic]]		[[Category:Material]] [[Category:Antibiotic]]

Christopher C Vanlang at 02:50, 2 January 2012

Christopher C Vanlang — Mon, 02 Jan 2012 02:50:47 GMT

←Older revision		Revision as of 02:50, 2 January 2012
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	==Plasmid stabilization==		==Plasmid stabilization==
	**Carbenicillin is used to select for cells bearing the beta-lactamase gene ''bla'' or ''ampR''. Beta-lactamase also neutralizes ampicillin, but due to ampicillin's lower effectiveness, carbenicillin tends to give fewer satellite colonies and makes for more reliable cloning. Ampicillin is more widely used, though, due to carbenicillin's high cost ($75/g compared to ampicillin's $10/g (Sigma)).		**Carbenicillin is used to select for cells bearing the beta-lactamase gene ''bla'' or ''ampR''. Beta-lactamase also neutralizes ampicillin, but due to ampicillin's lower effectiveness, carbenicillin tends to give fewer satellite colonies and makes for more reliable cloning. Ampicillin is more widely used, though, due to carbenicillin's high cost ($75/g compared to ampicillin's $10/g (Sigma)).
		+
		+	[[Category:Material]] [[Category:Antibiotic]]

Felix Moser: New page: ==Plasmid stabilization== **Carbenicillin is used to select for cells bearing the beta-lactamase gene ''bla'' or ''ampR''. Beta-lactamase also neutralizes ampicillin, but due to ampicillin...

Felix Moser — Wed, 05 Aug 2009 18:34:03 GMT

New page: ==Plasmid stabilization== **Carbenicillin is used to select for cells bearing the beta-lactamase gene ''bla'' or ''ampR''. Beta-lactamase also neutralizes ampicillin, but due to ampicillin...

New page

==Plasmid stabilization==
**Carbenicillin is used to select for cells bearing the beta-lactamase gene ''bla'' or ''ampR''. Beta-lactamase also neutralizes ampicillin, but due to ampicillin's lower effectiveness, carbenicillin tends to give fewer satellite colonies and makes for more reliable cloning. Ampicillin is more widely used, though, due to carbenicillin's high cost ($75/g compared to ampicillin's $10/g (Sigma)).