CTR:Recipes: Difference between revisions
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Lab recipes for general-use buffers. Also refer to the index card box located near the gel rigs. | Lab recipes for general-use buffers. Also refer to the index card box located near the gel rigs. | ||
==Queen's Lysis Buffer== | |||
*1.21g Tris Base | |||
*0.58g NaCl | |||
*3.73g EDTA | |||
*10.0g N-lauroylsarcosine | |||
*dH<sub>2</sub>O to 1L total volume | |||
==TAE Buffer (50x)== | |||
*242g Tris Base | |||
*23.8g EDTA (or 100mL 0.5M EDTA pH 8.0) | |||
*57.1mL glacial acetic acid | |||
*dH<sub>2</sub>O to 1L total volume | |||
==TBE Buffer (5x)== | |||
*54g Tris Base | |||
*27.5g Boric acid | |||
*3.72g EDTA (or 20mL 0.5M EDTA pH 8.0) | |||
*dH<sub>2</sub>O to 1L total volume | |||
==TE Buffer== | |||
*10mM TrisCl of desired pH | |||
*1mM EDTA pH 8.0 | |||
==LowTE Buffer== | |||
*10mM TrisHCl | |||
*0.1mM EDTA pH 8.0 | |||
example to make 15mL: | |||
*150μL TrisHCl 1M | |||
*3μL EDTA 0.5M | |||
*14.85mL dH<sub>2</sub>O | |||
Revision as of 16:38, 6 June 2014
Lab recipes for general-use buffers. Also refer to the index card box located near the gel rigs.
Queen's Lysis Buffer
- 1.21g Tris Base
- 0.58g NaCl
- 3.73g EDTA
- 10.0g N-lauroylsarcosine
- dH2O to 1L total volume
TAE Buffer (50x)
- 242g Tris Base
- 23.8g EDTA (or 100mL 0.5M EDTA pH 8.0)
- 57.1mL glacial acetic acid
- dH2O to 1L total volume
TBE Buffer (5x)
- 54g Tris Base
- 27.5g Boric acid
- 3.72g EDTA (or 20mL 0.5M EDTA pH 8.0)
- dH2O to 1L total volume
TE Buffer
- 10mM TrisCl of desired pH
- 1mM EDTA pH 8.0
LowTE Buffer
- 10mM TrisHCl
- 0.1mM EDTA pH 8.0
example to make 15mL:
- 150μL TrisHCl 1M
- 3μL EDTA 0.5M
- 14.85mL dH2O
