CTR:Notebook/PIRE/2015/08/25: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PIRE RAD Library Preps</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PIRE RAD Library Preps</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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*** 1 uL P1 adapter primer (25 uM) | *** 1 uL P1 adapter primer (25 uM) | ||
*** 1 uL P2 adapter primer (25 uM) | *** 1 uL P2 adapter primer (25 uM) | ||
[[Image:PCRproduct08272015 2.JPG|200px]] | |||
1) 2 uL 100 bp low scale ladder, 2) 1 uL DNA template, 3) 5 uL PCR product<br> | |||
→Successful amplification. | |||
*Clean up (2015-09-02, with Mice Plate 1) | |||
**45μL beads | |||
**Added a second cleanup using ~28μL beads | |||
**Ran gel to see final product | |||
[[Image:Libraries-skinksP3 miceP1.jpg|200px]] | |||
2μL Skink library on left | |||
*Picogreen: 1.34 ng/μL | |||
*Run 3μL on BioAnalyzer DNA High Sensitivity Chip (2015-09-03) | |||
[[Image:BioAnalyzer SkinkP3 2015-09-03.jpg|550px]] | |||
*Run 3μL on BioAnalyzer DNA High Sensitivity Chip (2015-) | |||
[[Image: | |||
*Sent 10nM to Berkeley for sequencing (Illumina HiSeq 100 PE) (2015-09-03) | |||
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Latest revision as of 01:05, 27 September 2017
PIRE RAD Library Preps | Main project page Previous entry Next entry |
RAD Library Prep - Skinks Plate 3 (48 wells, Reruns)
1) 2uL 100bp low scale ladder,
2) 2uL sheared DNA
1) 2uL 100bp low scale ladder, 2) 2uL sheared DNA
1) 2 uL 100 bp low scale ladder, 2) 1 uL DNA template, 3) 5 uL PCR product →Visible amplification, but size range difficult to discern.
1) 2 uL 100 bp low scale ladder, 2) 1 uL DNA template, 3) 5 uL PCR product
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