CTR:Notebook/PIRE/2015/04/01: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PIRE RAD Library Preps</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PIRE RAD Library Preps</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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3) 1uL of template | 3) 1uL of template | ||
*Qubit: 8.74 ng/uL | |||
*2nd PCR amplification (2015-05-01) | |||
**Used 10 uL of template | |||
**NEBPCR on JOHN Block A | |||
[[Image:Skinks2 pcr2 2015-05-01.jpg|100px]] | |||
<br> | |||
1) 2uL 100bp low scale ladder, | |||
2) 5uL of PCR product | |||
*Bead clean up | |||
**Use 45 uL AMPure beads (1:1) | |||
**2 washes of 200 uL 80% EtOH | |||
**Elute in 30 uL LowTE | |||
*Picogreen: 5.43 ng/uL | |||
*Qubit: 9.82 ng/uL | |||
*Ran 3μL on BioAnalyzer DNA High Sensitivity Chip (2015-02-06) | |||
[[Image:Screen Shot 2015-05-06 at 2.48.57 PM.png|650px]] | |||
Latest revision as of 00:52, 27 September 2017
PIRE RAD Library Preps | Main project page Previous entry Next entry |
RAD Library Prep - Skinks Plate 2
1) 2uL 100bp low scale ladder,
2) 2uL sheared DNA
1) 1 uL DNA template, 2) 5 uL PCR product, 3) 2 μL 100bp ladder
1) 5 uL PCR product, 2) 1 μL template, 3) 2 μL 100bp ladder
RAD Library Re-prep - Skinks Plate 2Reprep from plate.
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