CTR:Notebook/PIRE/2015/01/14: Difference between revisions
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*Quant DNA library using PicoGreen: 1.53 ng/μL. Using Qubit HS: 2.5 ng/μL. <b>→ Too low to get 10nm in 10μL.</b> | *Quant DNA library using PicoGreen: 1.53 ng/μL. Using Qubit HS: 2.5 ng/μL. <b>→ Too low to get 10nm in 10μL.</b> | ||
*Re-run of PCR using 10μL template DNA | *Re-run of PCR using 10μL template DNA (2015-01-16) | ||
**Mix: | **Mix: | ||
***10 uL DNA | ***10 uL DNA | ||
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*** 1 uL P2 adapter primer (25 uM) | *** 1 uL P2 adapter primer (25 uM) | ||
**PCR cycle (NEBPCR on JOHN Block B) | **PCR cycle (NEBPCR on JOHN Block B) | ||
[[Image:Skinks pcr 2015-01-16.JPG|200px]] | |||
1) 2 uL 100 bp low scale ladder, 2) 1 uL DNA template, 3) 5 uL PCR product | |||
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Revision as of 17:23, 16 January 2015
PIRE RAD Library Preps | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
RAD Library Prep - Skinks Plate 1
1) 2uL 100bp low scale ladder,
2) 2uL sheared DNA
1) 2 uL 100 bp low scale ladder, 2) 1 uL DNA template, 3) 5 uL PCR product
1) 2 uL 100 bp low scale ladder, 2) 1 uL DNA template, 3) 5 uL PCR product run (<3 ng/uL) on BioAnalyzer DNA High Sensitivity Chip
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