CTR:Notebook/PIRE/2015/01/14: Difference between revisions
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**Take 5 uL from each well and combine to 1.7 mL tube | **Take 5 uL from each well and combine to 1.7 mL tube | ||
**AMPure bead clean up: | **AMPure bead clean up: | ||
*** | ***Used 440 uL beads to DNA (1:1) | ||
***2 washes of 800 uL 80% EtOH | ***2 washes of 800 uL 80% EtOH | ||
***Elute in 100 uL LowTE | ***Elute in 100 uL LowTE | ||
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**Elute in 30 uL LowTE | **Elute in 30 uL LowTE | ||
*Quant DNA library using PicoGreen: 1.53 ng/μL. Using Qubit HS: 2.5 ng/μL. → Too low to get 10nm in 10μL. | *Quant DNA library using PicoGreen: 1.53 ng/μL. Using Qubit HS: 2.5 ng/μL. <b>→ Too low to get 10nm in 10μL.</b> | ||
*Re-run of PCR using 10μL template DNA | *Re-run of PCR using 10μL template DNA |
Revision as of 12:49, 16 January 2015
PIRE RAD Library Preps | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
RAD Library Prep - Skinks Plate 1
1) 2uL 100bp low scale ladder,
2) 2uL sheared DNA
1) 2 uL 100 bp low scale ladder, 2) 1 uL DNA template, 3) 5 uL PCR product
run (<3 ng/uL) on BioAnalyzer DNA High Sensitivity Chip
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