CTR:Notebook/PIRE/2015/01/14: Difference between revisions
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Sirena Lao (talk | contribs) |
Sirena Lao (talk | contribs) |
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***Elute in 20 uL LowTE | ***Elute in 20 uL LowTE | ||
*PCR amplification (2015-01- | *PCR amplification (2015-01-15) | ||
**Mix: | **Mix: | ||
***5 uL DNA | ***5 uL DNA | ||
Line 87: | Line 87: | ||
****72 degrees for 30 seconds | ****72 degrees for 30 seconds | ||
***72 degrees for 5 minutes | ***72 degrees for 5 minutes | ||
[[Image: | [[Image:Skinks pcr 2015-01-15.JPG|200px]] | ||
1 | 1) 2 uL 100 bp low scale ladder, 2) 1 uL DNA template, 3) 5 uL PCR product | ||
*Bead clean up (2015-01- | *Bead clean up (2015-01-16) | ||
**Use 45 uL AMPure beads (1:1) | **Use 45 uL AMPure beads (1:1) | ||
**2 washes of 200 uL 80% EtOH | **2 washes of 200 uL 80% EtOH |
Revision as of 17:40, 15 January 2015
PIRE RAD Library Preps | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
RAD Library Prep - Skinks Plate 1
1) 2uL 100bp low scale ladder,
2) 2uL sheared DNA
1) 2 uL 100 bp low scale ladder, 2) 1 uL DNA template, 3) 5 uL PCR product
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