CTR:Notebook/PIRE/2015/01/14: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Sirena Lao (talk | contribs) |
Sirena Lao (talk | contribs) |
||
Line 19: | Line 19: | ||
* P1 adapter ligation (2015-01-14) | * P1 adapter ligation (2015-01-14) | ||
**Add 2 uL indexed P1 adapter (10nM) to each well | **Add 2 uL indexed P1 adapter (10nM) to each well, using new plate of adapters made in-house | ||
**Mix and add to each well: | **Mix and add to each well: | ||
***1.28 uL water | ***1.28 uL water |
Revision as of 12:08, 14 January 2015
PIRE RAD Library Preps | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
RAD Library Prep - Skinks Plate 1
1) 1uL pre-sheared DNA, 2) 2uL 100bp low scale ladder, 3) 3uL sheared DNA
1) 1 uL DNA template, 2) 2 uL 100 bp low scale ladder, 3) 5 uL PCR product
|