CTR:Notebook/PIRE/2014/11/21: Difference between revisions

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==RAD P1 Adapter Annealing and Dilution==
==RAD P1 Adapter Annealing and Dilution==


==RAD Library Prep - ==
*


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Revision as of 14:06, 21 November 2014

PIRE RAD Library Preps <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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RAD P1 Adapter Ordering

Adapter Sequences (sequences from Mike Miller's files)
Ordered in plate format from IDT (1 plate of top oligos, 1 plate of bottom oligos)
Parameters:

  • Scale: 100 nmole
  • Purification: Standard desalting
  • Ship option: Wet
  • Amount per well: Full yield
  • Concentration: 50 μM
  • Dilutant: IDTE Buffer pH 8.0

RAD P1 Adapter Annealing and Dilution
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