CTR:Notebook/PIRE/2014/11/21: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PIRE RAD Adapter | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PIRE RAD Adapter Preparation </span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Latest revision as of 00:32, 27 September 2017
PIRE RAD Adapter Preparation | Main project page Previous entry Next entry |
RAD P1 Adapter OrderingAdapter Sequences (sequences from Mike Miller's files)
RAD P1 Adapter Annealing and DilutionBased on instructions from Mike Miller's RAD adapter information file. 1. Dilute oligo plates from 100μM to 10μM
2.Make adapter mix plate (20μL)
3. Anneal adapters
4. Make dilution plate (195μL of 10mM Tris, 50mM NaCl)
5. Make final adapter plates (10nM)
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